Goat anti-Ferret IgG (Chain gamma) Antibody (HRP)

Details for Product No. ABIN117102, Supplier: Log in to see
Antigen
Epitope
Chain gamma
4680
2536
1881
1066
1006
860
300
293
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Ferret
2849
2739
2297
1675
1433
788
613
442
380
379
358
339
291
290
208
138
135
122
41
28
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Goat
6707
4832
1795
891
609
388
91
55
52
21
8
5
5
5
2
1
1
Clonality
Polyclonal
Conjugate
HRP
2234
1872
1744
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
48
46
43
40
38
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
10
10
9
9
9
9
9
9
9
8
8
8
8
8
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Ferret IgG gamma (gamma chain)
Purification Prepared from monospecific antiserum by Immunoaffinity Chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Ferret Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/5,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL of deionized water or equivalent.
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Thimerisol as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -28 °C or below.
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).