Rabbit anti-Human IgG1 Antibody

Catalog No. ABIN1172190

Product Details

Target: IgG1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: The antibody is a rabbit polyclonal antibody raised against IgG1. It has been selected for its ability to recognize IgG1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: Native Protein IgG1

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 0.5-2 μg/mL
• Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
• Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
• Immunohistochemistry in paraffin section: 5-20 μg/mL
• Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
• Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

References

Shao, Fu, Liu, Wang, Ding, Wang, Li, Shao: "IgG autoantibody subclasses altered in immuno-related hemocytopenia." in: Cellular immunology, Vol. 294, Issue 1, pp. 13-20, (2015) (PubMed).

Target Details

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody

Background: Alternative Names: IGHG1, Ig Gamma-1 Chain C Region, Immunoglobulin Heavy Constant Gamma 1, G1m Marker, Immunoglobulin Gm1