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Rabbit anti-Human IgG (Whole Molecule) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB, DB, IM Polyclonal Biotin

Catalog No. ABIN117247

Datasheet as PDF

Target See all IgG products

IgG
Binding Specificity
2333

499

411

188

129

122

92

29

28

28

15

9

6

6

6

6

5

2

2

1

1

1

1
Whole Molecule
Reactivity
692

672

558

451

366

239

198

147

137

131

122

98

95

95

92

62

46

33

27

17

12

10

8

5

5

4

3

3

3

3

3

3

3

3

2

2

2

1

1

1

1
Human
Host
1716

1474

537

203

118

103

33

31

21

17

15

11

11

1
Rabbit
Clonality
3347

164

1
Polyclonal
Conjugate
556

408

406

367

298

276

228

120

99

67

66

62

62

52

48

47

44

43

43

41

39

32

32

32

31

30

29

29

28

28

28

28

28

28

28

28

28

27

26

26

25

21

19

17

17

16

16

15

15

14

10
Biotin
Application
1837

1789

1785

1499

960

934

913

515

234

115

115

111

100

77

59

50

50

49

32

27

25

21

17

16

11

8

5

4

4

3

2

2

2

2

2

1

1

1

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunomicroscopy (IM)

Characteristics

Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.

Purification

Preadsorption: Solid phase absorption

Immunogen

Human IgG, whole molecule.
Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Human IgG (Heavy & Light Chain) antibody (Biotin) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Biotin

Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Human IgG (F(ab')2 Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Mouse anti-Human IgG (Heavy & Light Chain) antibody
ELISA, WB Monoclonal 4D2D9G8-4A10 IgG1 unconjugated

Mouse anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP

Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated

Rabbit anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Human IgG (Fc Region) antibody (HRP)
ELISA, WB Polyclonal HRP F(ab')2 fragment

Application Notes

Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:17,000 to 1:75,000 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore with 1.0 mL of deionized water (or equivalent).

Concentration

2.0 mg/mL

Buffer

0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Thimerisol as preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

Biohazard Informations: This product contains thimerosal which is hazardous.

Handling Advice

Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.

Storage

4 °C/-20 °C

Storage Comment

Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target

IgG

Abstract

IgG Products

Target Type

Antibody

Background

Synonyms: Human Immunoglobulin G