Goat anti-Human IgA, IgG, IgM (Whole Molecule) Antibody (Biotin)

Details for Product No. ABIN117310, Supplier: Log in to see
Antigen
Epitope
Whole Molecule
106
78
52
52
30
11
Reactivity
Human
229
172
79
60
29
26
10
8
7
7
6
5
3
3
3
Host
Goat
506
107
12
8
4
2
2
Clonality
Polyclonal
Conjugate
Biotin
103
103
80
48
33
32
18
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen Human IgG, IgA and IgM whole molecules
Characteristics Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
Purification Immunoaffinity chromatography
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Human IgA, Human IgG, Human IgM
Application Notes Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISAusing Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid) as a substrate for 30 minutes at roomtemperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL deionized water or equivalent.
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).