Acetylcholinesterase antibody (AA 157-332)

Catalog No. ABIN1174628

Product Details anti-Acetylcholinesterase Antibody

Target: Acetylcholinesterase (AChE)

Binding Specificity: AA 157-332

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Acetylcholinesterase (ACHE)

Specificity: The antibody is a rabbit polyclonal antibody raised against ACHE. It has been selected for its ability to recognize ACHE in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Acetylcholinesterase (ACHE) corresdonding to Gly157~Pro332 (Accession # P37136)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:300-1300 Immunohistochemistry: 5-20 μg/mL

1:30-130 Immunocytochemistry: 5-20 μg/mL

1:30-130 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-Acetylcholinesterase antibody (ABIN1174628)

Liu, Zhang, Ma, Song, Liu, Wang, Li, Yang, Yu: "Feishu Acupuncture Inhibits Acetylcholine Synthesis and Restores Muscarinic Acetylcholine Receptor M2 Expression in the Lung When Treating Allergic Asthma." in: Inflammation, Vol. 41, Issue 3, pp. 741-750, (2018) (PubMed).

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: Acetylcholinesterase (AChE Products)

Synonyms: ACEE antibody, ARACHE antibody, N-ACHE antibody, YT antibody, Ache antibody, GB14873 antibody, zgc:92550 antibody, ACE antibody, Dsim\\GD20515 antibody, GD20515 antibody, dsim_GLEANR_4292 antibody, ache antibody, mE1a antibody, mE1b antibody, mE1c antibody, mE1c-long antibody, mE1d antibody, mE1d' antibody, mE1e antibody, arache antibody, n-ache antibody, acetylcholinesterase (Cartwright blood group) antibody, acetylcholinesterase 2 antibody, acetylcholinesterase antibody, Acetylcholine esterase antibody, acetylcholinesterase (Cartwright blood group) L homeolog antibody, collagen type I alpha 2 chain antibody, ACHE antibody, AChE-2 antibody, Ache antibody, ache antibody, Dsim\Ace antibody, ache.L antibody, COL1A2 antibody, ACE-1 antibody

Background: ARAChE, N-AChE, YT

Pathways: Skeletal Muscle Fiber Development