PI3 antibody (AA 41-117) (Biotin)
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- Target See all PI3 Antibodies
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
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Binding Specificity
- AA 41-117
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PI3 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Sequence
- MGHHHHHHSG SEF-FNGQDPVKGQ VSVKGQDKVK AQEPVKGPVS TKPGSCPIIL IRCAMLNPPN RCLKDTDCPG IKKCCEGSCG MACFVPQ
- Specificity
- It has been selected for its ability to recognize PI3 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant PI3 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against PI3 conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product PI3 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant PI3 (Phe41~Gln117) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))
- Alternative Name
- Peptidase Inhibitor 3, Skin Derived (PI3) (PI3 Products)
- Synonyms
- ESI antibody, SKALP antibody, WAP3 antibody, WFDC14 antibody, cementoin antibody, trappin-2 antibody, TRAPPIN-2 antibody, elafin antibody, peptidase inhibitor 3 antibody, peptidase inhibitor 3, skin-derived (SKALP) antibody, PI3 antibody
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