Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN117684

Goat anti-Human IgG (Fc Region) Antibody (FITC)

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
»Alternatives Fc Region
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Conjugate
Pubmed 1 reference available
Catalog no. ABIN117684
Quantity 1 mg
Price
280.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Human IgG F(c) fragment
Fragment F(ab')2 fragment
Specificity This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti- Fluorescein, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab’)2 or Bovine, Horse, Mouse and Rat Serum Proteins. Species Reactivity: Tested: Human.
Characteristics Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 3.0 moles FITC per mole of Goat IgG F(ab')2
Purification Immunoaffinity Chromatography.
Background Alternate names: Human Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence ofF(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1.0 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid.
Expiry Date 12 months
The, Feltkamp: "Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation." in: Immunology, Vol. 18, Issue 6, pp. 865-73, 1970 (PubMed).

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts (3908), (2738), (1027), (379), (209), (208), (29), (26), (14), (6), (3), (3), (2), (1), (1)
Reactivities (1575), (1384), (1282), (1023), (786), (406), (351), (229), (227), (204), (188), (184), (179), (175), (89), (76), (62), (53), (32), (31), (18), (12), (6), (5), (4), (3), (3), (1)
Conjugates (1311), (1200), (1020), (853), (582), (412), (367), (201), (199), (116), (115), (113), (111), (101), (84), (31), (27), (20), (20), (17), (15), (13), (13), (10), (10), (10), (10), (10), (10), (10), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4078), (940), (683), (143), (67), (65), (40), (20), (14), (14), (14), (14), (4), (4), (2), (1)
Beacon