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Goat anti-Human IgG (Fc Region) Antibody (FITC)

Details for Product No. ABIN117684, Supplier: Log in to see
Antigen
Epitope
Fc Region
4276
1867
1731
962
643
277
203
56
35
34
31
8
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
2413
2318
2036
1524
1260
609
562
382
328
296
296
291
290
224
162
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Goat
6020
4115
1527
600
545
262
85
49
43
8
8
4
4
3
1
1
Clonality
Polyclonal
Conjugate
FITC
1870
1683
1511
1095
605
523
517
261
198
181
172
161
156
155
127
79
79
69
43
41
37
32
32
31
23
20
19
19
17
15
14
14
14
14
13
10
10
10
10
10
10
9
9
9
9
8
8
8
8
8
8
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunomicroscopy (IM)
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Immunogen Human IgG F(c) fragment
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Molar Ratio: 3.0 moles FITC per mole of Goat IgG F(ab')2
Purification Immunoaffinity Chromatography.
This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Background Synonyms: Human Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment

Absorption / Emission: 495 nm / 528 nm

Restrictions For Research Use only
Reconstitution Restore with 1.0 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Background publications The, Feltkamp: "Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation." in: Immunology, Vol. 18, Issue 6, pp. 865-73, 1970 (PubMed).