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Goat anti-Human IgG (Fc Region) Antibody (FITC)

Details for Product No. ABIN117684
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Antigen
Epitope
Fc Region
(3561), (2017), (1426), (1035), (763), (229), (189), (73), (33), (32), (29), (7), (5), (2), (2), (2), (2), (1), (1)
Reactivity
Human
(1887), (1869), (1541), (1214), (972), (497), (435), (343), (297), (266), (266), (259), (225), (222), (121), (93), (85), (81), (32), (28), (21), (14), (8), (8), (7), (5), (5), (4), (3), (3), (2), (1), (1), (1)
Host
Goat
(4786), (3557), (1182), (463), (395), (254), (63), (44), (25), (8), (5), (1)
Clonality
Polyclonal
Conjugate
FITC
(1510), (1377), (1246), (1003), (674), (553), (413), (201), (199), (116), (115), (113), (111), (107), (100), (33), (32), (21), (20), (15), (11), (10), (10), (10), (10), (10), (10), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunomicroscopy (IM), Flow Cytometry (FACS)
Pubmed 1 reference available
Quantity 1 mg
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Catalog No. ABIN117684
291.50 $
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Immunogen Human IgG F(c) fragment
Specificity Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti- Fluorescein, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.
Characteristics Molar Ratio: 3.0 moles FITC per mole of Goat IgG F(ab')2
Purification Immunoaffinity Chromatography.
This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Background Synonyms: Human Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment

Absorption / Emission: 495 nm / 528 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1.0 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Expiry Date 12 months
General The, Feltkamp: "Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation." in: Immunology, Vol. 18, Issue 6, pp. 865-73, 1970 (PubMed).

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