ITGA4 antibody

Catalog No. ABIN1176851

Product Details anti-ITGA4 Antibody

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: AKR/Cum mouse spontaneous T lymphoma line TK1

Clone: R1-2

Isotype: IgG2b kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-ITGA4 antibody (ABIN1176851)

Brocke, Piercy, Steinman, Weissman, Veromaa: "Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6896-901, (1999) (PubMed).

Chisholm, Williams, Lobb: "Monoclonal antibodies to the integrin alpha-4 subunit inhibit the murine contact hypersensitivity response." in: European journal of immunology, Vol. 23, Issue 3, pp. 682-8, (1993) (PubMed).

Ferguson, Kupper: "Antigen-independent processes in antigen-specific immunity. A role for alpha 4 integrin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1172-82, (1993) (PubMed).

Kilshaw, Murant: "Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system." in: European journal of immunology, Vol. 21, Issue 10, pp. 2591-7, (1991) (PubMed).

Holzmann, McIntyre, Weissman: "Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha." in: Cell, Vol. 56, Issue 1, pp. 37-46, (1989) (PubMed).

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d (ITGA4 Products)

Synonyms: CD49D antibody, IA4 antibody, LOC100219853 antibody, integrin subunit alpha 4 antibody, integrin alpha 4 antibody, integrin subunit alpha 4 S homeolog antibody, ITGA4 antibody, Itga4 antibody, itga4.S antibody

Background: The R1-2 antibody reacts with the integrin alpha4 chain (CD49d), which is expressed as a heterdimer with either of two beta, beta1 or beta7 (also known as betap). The alpha4beta1 integrin (VLA-4, CD49d/CD29) is expressed on most peripheral lymphocytes, thymocytes, and monocytes, while the alpha4beta7 integrin (LPAM-1) is expressed on peripheral lymphocytes, but on only a small subset of thymocytes. These integrins mediate a variety of cell-cell and cell-matrix interactions, recognizing the ligands VCAM-1 (CD106) and fibronectin. There is evidence that levels of VLA-4 expression regulate the transendothelial migration of T lymphocytes into inflamed tissues. Integrin alpha4beta7 also preferentially binds to the mucosal vascular addressin, MAdCAM-1. The R1-2 antibody blocks some alpha4 integrin-mediated binding functions. In combination with mAb 9C10 (MFR4.B) (Cat. No. 553313), binding of VLA-4 expressing cells to VCAM-1 can be almost completely inhibited.
Synonyms: Integrin alpha4 chain

Pathways: Integrin Complex