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IL7R antibody

IL7R Reactivity: Mouse FACS, WB, IHC, IP, BR Host: Rat Monoclonal SB-14 unconjugated
Catalog No. ABIN1176855
  • Target See all IL7R Antibodies
    IL7R (Interleukin 7 Receptor (IL7R))
    Reactivity
    • 110
    • 50
    • 26
    • 7
    • 4
    • 2
    • 1
    Mouse
    Host
    • 81
    • 36
    • 28
    • 1
    • 1
    Rat
    Clonality
    • 81
    • 64
    • 2
    Monoclonal
    Conjugate
    • 63
    • 11
    • 10
    • 8
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL7R antibody is un-conjugated
    Application
    • 85
    • 77
    • 45
    • 26
    • 26
    • 13
    • 13
    • 9
    • 8
    • 8
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Blocking Reagent (BR)
    Brand
    BD Pharmingen™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
    Immunogen
    BALB/c mouse pre-B cell line 1A9
    Clone
    SB-14
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product IL7R Primary Antibody
  • Application Notes
    Flow cytometry: Since this antigen is expressed at low density on lymphoid cell surfaces, it may be desirable to amplify staining by using a bright second-step reagent. If a three-step method is employed, the use of anti-Mouse CD16/CD32, Mouse Fc Block™ (Cat. No. 553141) is recommended to reduce non-specific staining. Special precaution should be taken to utilize a second-step antibody which does not crossreact with 2.4G2 mAb (rat IgG2b, kappa), such as biotinylated anti-rat IgG2a (RG7/1.30, Cat. No. 553894) followed by PE Streptavidin (Cat. No. 554061).
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
    Preservative
    Azide free
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • Akashi, Kondo, Weissman: "Role of interleukin-7 in T-cell development from hematopoietic stem cells." in: Immunological reviews, Vol. 165, pp. 13-28, (1999) (PubMed).

    Borghesi, Yamashita, Kincade: "Heparan sulfate proteoglycans mediate interleukin-7-dependent B lymphopoiesis." in: Blood, Vol. 93, Issue 1, pp. 140-8, (1999) (PubMed).

    Fujihashi, Kawabata, Hiroi, Yamamoto, McGhee, Nishikawa, Kiyono: "Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3613-8, (1996) (PubMed).

    Noguchi, Nakamura, Russell, Ziegler, Tsang, Cao, Leonard: "Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1877-80, (1994) (PubMed).

    Peschon, Morrissey, Grabstein, Ramsdell, Maraskovsky, Gliniak, Park, Ziegler, Williams, Ware, Meyer, Davison: "Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1955-60, (1994) (PubMed).

    Faust, Saffran, Toksoz, Williams, Witte: "Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells." in: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 915-23, (1993) (PubMed).

    Sudo, Nishikawa, Ohno, Akiyama, Tamakoshi, Yoshida, Nishikawa: "Expression and function of the interleukin 7 receptor in murine lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 9125-9, (1993) (PubMed).

    Henderson, Narayanan, Collins, Dorshkind: "Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 1973-9, (1992) (PubMed).

    Goodwin, Friend, Ziegler, Jerzy, Falk, Gimpel, Cosman, Dower, March, Namen: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." in: Cell, Vol. 60, Issue 6, pp. 941-51, (1990) (PubMed).

  • Target
    IL7R (Interleukin 7 Receptor (IL7R))
    Alternative Name
    CD127 (IL7R Products)
    Synonyms
    IL7R antibody, CD127 antibody, IL-7Ralpha antibody, CDW127 antibody, IL-7R-alpha antibody, IL7RA antibody, ILRA antibody, IL-7RA antibody, interleukin 7 receptor antibody, IL7R antibody, Il7r antibody
    Background
    The SB/14 monoclonal antibody reacts with CD127, the 65-75-kDa type-I transmembrane protein IL-7Ralpha. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Ralpha and CD132, the common gamma chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4-CD8- immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities. IL-7Ralpha is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density gammadelta TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring alphabeta TCR-bearing T cells. The SB/14 mAb neutralizes IL-7-induced proliferation of the IL-7-dependent immature B-lymphocyte line 2E8 (detected by BrdU incorporation). Blocking of IL-7-induced lymphocyte proliferation. 2E8 cells (mouse immature B lymphocyte cell line) were cultured for 48 hours in the presence of recombinant mouse IL-7 (blue histogram), with mAb SB/14 added 30 minutes prior to IL-7 (red histogram), or in the absence of IL-7 (green histogram). Proliferation was detected by incorporation of bromodeoxyuridine (BrdU) using the BrdU Flow Kit, Cat. No. 559619. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
    Synonyms: IL-7 Receptor alpha chain
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
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