CD31 antibody

Catalog No. ABIN1176859

Product Details anti-CD31 Antibody

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D

Clone: 390

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD31 antibody (ABIN1176859)

Duncan, Andrew, Takimoto, Kaufman, Yoshida, Spellberg, de la Pompa, Elia, Wakeham, Karan-Tamir, Muller, Senaldi, Zukowski, Mak: "Genetic evidence for functional redundancy of Platelet/Endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 3022-30, (1999) (PubMed).

Horenstein, Stockinger, Imhof, Malavasi: "CD38 binding to human myeloid cells is mediated by mouse and human CD31." in: The Biochemical journal, Vol. 330 ( Pt 3), pp. 1129-35, (1998) (PubMed).

Buck, Edelman, Buck, Kennedy, Baldwin: "Expression patterns of adhesion receptors in the developing mouse lung: functional implications." in: Cell adhesion and communication, Vol. 4, Issue 2, pp. 69-87, (1997) (PubMed).

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, (1997) (PubMed).

Famiglietti, Sun, DeLisser, Albelda: "Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity." in: The Journal of cell biology, Vol. 138, Issue 6, pp. 1425-35, (1997) (PubMed).

Iguchi, Okuyama, Koguma, Obinata, Yanai: "Selective stimulation of granulopoiesis in vitro by established bone marrow stromal cells." in: Cell structure and function, Vol. 22, Issue 3, pp. 357-64, (1997) (PubMed).

Ling, Luxenberg, Wang, Nickbarg, Leenen, Neben, Kobayashi: "Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31)." in: European journal of immunology, Vol. 27, Issue 2, pp. 509-14, (1997) (PubMed).

Rosenblum, Nelson, Wormley, Werner, Wang, Shih: "Role of platelet-endothelial cell adhesion molecule (PECAM) in platelet adhesion/aggregation over injured but not denuded endothelium in vivo and ex vivo." in: Stroke; a journal of cerebral circulation, Vol. 27, Issue 4, pp. 709-11, (1996) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

Buck, Baldwin, DeLisser, Mickanin, Shen, Kennedy, Chen, Edelman, Albelda: "Cell adhesion receptors and early mammalian heart development: an overview." in: Comptes rendus de l'Acade?mie des sciences. Se?rie III, Sciences de la vie, Vol. 316, Issue 9, pp. 838-59, (1994) (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, (1994) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 (PECAM-1) (PECAM1 Products)

Synonyms: CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody

Background: The 390 monoclonal antibody specifically binds to CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ~130 kDa integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is weakly expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development, this alternative splicing is involved in regulation of ligand specificity. CD38 and vitronectin receptor (alphavbeta3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The 390 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.
Synonyms: Platelet endothelial cell adhesion molecule, Pecam1, PECAM-1, Pecam-1

Pathways: Regulation of Actin Filament Polymerization