ERK2 antibody (Mitogen-Activated Protein Kinase 1) (Alexa Fluor 488)

Details for Product anti-MAPK1 Antibody No. ABIN1176894, Supplier: Log in to see
Antigen
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • ATMPK2
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • T30E16.13
  • T30E16_13
  • mitogen-activated protein kinase homolog 2
  • ERK2
  • zERK2
  • MAPK1
  • ERK
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • erk
  • erk2
  • ert1
  • mapk
  • mpk1
  • xp42
  • mapk2
  • prkm1
  • prkm2
  • Xp42
  • mapk1b
  • mapk1
  • mapk1a
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 2
  • mitogen activated protein kinase 1
  • ATMPK1
  • MPK1
  • MPK2
  • MAPK1
  • mapk1
  • Mapk1
  • PfMAP1
  • PVX_084965
  • Tsp_01601
  • mapk1-b
  • mapk1-a
Alternatives
anti-Human ERK2 antibody for Western Blotting
Reactivity
Human, Mouse (Murine)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This ERK2 antibody is conjugated to Alexa Fluor 488
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Application
Intracellular Staining (ICS)
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Options
Supplier
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Supplier Product No.
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Brand BD Phosflow™
Immunogen Human ERK2 Synthetic Peptide
Clone G263-7
Isotype IgG1, kappa
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases, ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other cytoplasmic signaling molecules (protein kinases and phosphatases), cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers. The G263-7 recognizes ERK2. It does not cross-react with ERK1. Clone G263-7 was originally characterized in human (A431) and mouse (NIH/3T3) cells. A human ERK2 synthetic peptide was used as immunogen.
Research Area Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals
Sample Volume 20 μL
Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Product cited in: Sweatt: "The neuronal MAP kinase cascade: a biochemical signal integration system subserving synaptic plasticity and memory." in: Journal of neurochemistry, Vol. 76, Issue 1, pp. 1-10, 2001 (PubMed).

Sivaraman, Wang, Nuovo, Malbon: "Hyperexpression of mitogen-activated protein kinase in human breast cancer." in: The Journal of clinical investigation, Vol. 99, Issue 7, pp. 1478-83, 1997 (PubMed).

Treisman: "Regulation of transcription by MAP kinase cascades." in: Current opinion in cell biology, Vol. 8, Issue 2, pp. 205-15, 1996 (PubMed).

Davis: "The mitogen-activated protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 268, Issue 20, pp. 14553-6, 1993 (PubMed).

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