BTK antibody (N-Term) (PE)

Catalog No. ABIN1176914

Product Details anti-BTK Antibody (PE)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Binding Specificity: AA 2-172, N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BTK antibody is conjugated to PE

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human N-Terminal Btk aa. 2-172 Recombinant Protein

Clone: 53-BTK

Isotype: IgG2a

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-BTK antibody (PE) (ABIN1176914)

Marshall, Niiro, Yun, Clark: "Regulation of B-cell activation and differentiation by the phosphatidylinositol 3-kinase and phospholipase Cgamma pathway." in: Immunological reviews, Vol. 176, pp. 30-46, (2001) (PubMed).

Rawlings, Scharenberg, Park, Wahl, Lin, Kato, Fluckiger, Witte, Kinet: "Activation of BTK by a phosphorylation mechanism initiated by SRC family kinases." in: Science (New York, N.Y.), Vol. 271, Issue 5250, pp. 822-5, (1996) (PubMed).

Mahajan, Fargnoli, Burkhardt, Kut, Saouaf, Bolen: "Src family protein tyrosine kinases induce autoactivation of Bruton's tyrosine kinase." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5304-11, (1995) (PubMed).

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: Btk (BTK Products)

Synonyms: BTK antibody, atk antibody, bpk antibody, xla antibody, imd1 antibody, agmx1 antibody, psctk1 antibody, AGMX1 antibody, AT antibody, ATK antibody, BPK antibody, IMD1 antibody, PSCTK1 antibody, XLA antibody, AI528679 antibody, xid antibody, Bruton tyrosine kinase antibody, Bruton agammaglobulinemia tyrosine kinase antibody, BTK antibody, btk antibody, Btk antibody

Background: Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. It is a member of the Tec family of kinases which includes Tec and Itk. This family is similar to the src family of tyrosine kinases. However, Tec family members lack the N-terminal myristylation site and the regulatory C-terminal tyrosine that are found in src proteins. In addition to an N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80 amino acids between the PH and SH3 domains termed the Tec homology domain. The activity of Btk is regulated by Src-mediated phosphorylation of the kinase domain at tyrosine 551. This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine 223 in the SH3 domain. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The PH domain is essential for proper activation and function of Btk. A mutation in the PH domain results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia.

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling