OCT4 antibody (Isoform A) (Alexa Fluor 647)

Catalog No. ABIN1176919

Product Details anti-OCT4 Antibody (Alexa Fluor 647)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Binding Specificity: Isoform A

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is conjugated to Alexa Fluor 647

Application: Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Oct3/4 Isoform A Recombinant Protein

Clone: O50-808

Isotype: IgG1 kappa

Application Details

Comment:

BALB/c

Sample Volume: 5 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-OCT4 antibody (Alexa Fluor 647) (ABIN1176919)

Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, (2007) (PubMed).

Nishimoto, Fukushima, Okuda, Muramatsu: "The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5453-65, (1999) (PubMed).

Vigano, Staudt: "Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1." in: Nucleic acids research, Vol. 24, Issue 11, pp. 2112-8, (1996) (PubMed).

Rosfjord, Scholtz, Lewis, Rizzino: "Phosphorylation and DNA binding of the octamer binding transcription factor Oct-3." in: Biochemical and biophysical research communications, Vol. 212, Issue 3, pp. 847-53, (1995) (PubMed).

Yuan, Corbi, Basilico, Dailey: "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3." in: Genes & development, Vol. 9, Issue 21, pp. 2635-45, (1995) (PubMed).

Okamoto, Okazawa, Okuda, Sakai, Muramatsu, Hamada: "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells." in: Cell, Vol. 60, Issue 3, pp. 461-72, (1990) (PubMed).

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct3/4 (POU5F1 Products)

Synonyms: OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody

Background: Development of a multicellular organism from a single fertilized egg is regulated by the coordinated activity of DNA transcription factors. Oct3/4, a member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem (ES) cell lines and embryonal carcinomas (EC). The human POU5F1 gene can encode various splice variants, two of which are Oct3/4A and Oct3/4B. Both isoforms share identical POU DNA-binding and C-terminal domains but differ in their N-terminal domain. The N-terminal domain of Oct3/4B is inhibitory to the DNA binding domain and therefore cannot stimulate transcription of Oct3/4-dependent genes. Oct3/4B can be detected in both pluripotent and some differentiated cell types in both the nucleus and cytoplasm, but its function is unclear. There is not an equivalent to Oct3/4B in mouse. Oct3/4A is expressed in the nucleus and has been demonstrated to orchestrate the transcription of Oct3/4-dependent genes. It has been demonstrated that the expression of Oct3/4 isoforms can vary greatly in different cell types, and discrimination of these is crucial for assessing Oct3/4 expression and function. The O50-808 monoclonal antibody recognizes human Oct3/4 Isoform A and mouse Oct3/4.
Synonyms: Oct3/4A, Oct-3A, OTF-3, NF-A3, OTF4, POU5F1, MGC22487

Pathways: Stem Cell Maintenance