Selectin E/CD62e antibody

Catalog No. ABIN1176966

Product Details anti-Selectin E/CD62e Antibody

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse brain capillary endothelioma bEnd.3 (TNFalpha-stimulated)

Clone: 10E9-6

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-Selectin E/CD62e antibody (ABIN1176966)

Pendl, Robert, Steinert, Thanos, Eytner, Borges, Wild, Lowe, Fuhlbrigge, Kupper, Vestweber, Grabbe: "Immature mouse dendritic cells enter inflamed tissue, a process that requires E- and P-selectin, but not P-selectin glycoprotein ligand 1." in: Blood, Vol. 99, Issue 3, pp. 946-56, (2002) (PubMed).

Borges, Pendl, Eytner, Steegmaier, Zöllner, Vestweber: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).

Ramos, Kunkel, Lawrence, Jung, Vestweber, Bosse, McIntyre, Gillooly, Norton, Wolitzky, Ley: "Differential effect of E-selectin antibodies on neutrophil rolling and recruitment to inflammatory sites." in: Blood, Vol. 89, Issue 8, pp. 3009-18, (1997) (PubMed).

Eppihimer, Wolitzky, Anderson, Labow, Granger: "Heterogeneity of expression of E- and P-selectins in vivo." in: Circulation research, Vol. 79, Issue 3, pp. 560-9, (1996) (PubMed).

Bosse, Vestweber: "Only simultaneous blocking of the L- and P-selectin completely inhibits neutrophil migration into mouse peritoneum." in: European journal of immunology, Vol. 24, Issue 12, pp. 3019-24, (1995) (PubMed).

Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

Weller, Isenmann, Vestweber: "Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha." in: The Journal of biological chemistry, Vol. 267, Issue 21, pp. 15176-83, (1992) (PubMed).

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: CD62E (E-selectin) (SELE Products)

Background: The 10E9.6 antibody reacts with the 97-110 kDa cell surface glycoprotein E-selectin (CD62E), also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1), which is expressed on endotoxin- or cytokine-stimulated mouse endothelial cells. A suspension of TNFalpha stimulated mouse brain capillary endothelioma cells, from the cell line bEnd.3, was used as the immunogen. The epitope recognized by mAb 10E9.6 has been mapped to the first and/or second complement regulatory protein repeat domains of E-selectin. The 10E9.6 antibody has been reported to block binding of a monocyte cell line to E-selectin in vitro and to block neutrophil migration in BALB/c, but not C57BL/6 mice. It has no effect on leukocyte rolling in TNFalpha-treated mouse venules or on in vitro adhesion of myeloid cells to E-selectin. Studies have demonstrated that Cutaneous Lymphocyte Antigen (CLA), recognized by mAb HECA-452 (Cat. no. 555946), may be a ligand for CD62E.
Synonyms: E-selectin, ELAM-1

Pathways: Thromboxane A2 Receptor Signaling