CD28 antibody

Catalog No. ABIN1176978

Product Details anti-CD28 Antibody

Target: CD28

Reactivity: Mouse

Host: Golden Syrian Hamster

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Cytotoxicity Test (CyTox)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse EL-4 (T-cell lymphoma) Cells

Clone: 37-51

Isotype: IgG2 lambda

Application Details

Application Notes: Flow Cytometry: For multicolor staining of cell suspensions from peripheral lymphoid tissues (e.g detection of T lymphocytes and/or NK cells), investigators are encouraged to utilize Purified Rat Anti-Mouse CD16/CD32 [Mouse BD Fc Block™] (Cat. No. 553141) to minimize non-specific binding. In addition, since CD28 may be expressed at low density on resting peripheral T lymphocytes, investigators may wish to amplify the staining signal by using a biotinylated second-step antibody followed by a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061). If Mouse BD Fc Block™ is used, investigators need to be aware of and account for any potential crossreactivity that may occur between the second-step or third-step reagents chosen with the Mouse BD Fc Block™. Biotin Mouse Anti-Syrian Hamster IgG2 (Cat. No. 554029) would be a suggested second-step antibody for investigators to consider utilizing.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD28 antibody (ABIN1176978)

Marietta, Weis, Weis: "CD28 expression by mouse mast cells is modulated by lipopolysaccharide and outer surface protein A lipoprotein from Borrelia burgdorferi." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2840-8, (1997) (PubMed).

Ong, Lim, Teh: "CD28-induced cytokine production and proliferation by thymocytes are differentially regulated by the p59fyn tyrosine kinase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2169-76, (1997) (PubMed).

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Rakasz, Hagen, Sandor, Lynch: "Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules." in: International immunology, Vol. 9, Issue 1, pp. 161-7, (1997) (PubMed).

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, (1996) (PubMed).

Gelfanov, Lai, Gelfanova, Dong, Su, Liao: "Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 76-82, (1995) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, (1994) (PubMed).

Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, (1994) (PubMed).

Harding, Allison: "CD28-B7 interactions allow the induction of CD8+ cytotoxic T lymphocytes in the absence of exogenous help." in: The Journal of experimental medicine, Vol. 177, Issue 6, pp. 1791-6, (1993) (PubMed).

Shahinian, Pfeffer, Lee, Kündig, Kishihara, Wakeham, Kawai, Ohashi, Thompson, Mak: "Differential T cell costimulatory requirements in CD28-deficient mice." in: Science (New York, N.Y.), Vol. 261, Issue 5121, pp. 609-12, (1993) (PubMed).

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, (1992) (PubMed).

Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, (1992) (PubMed).

Target Details for CD28

Target: CD28

Alternative Name: CD28 (CD28 Products)

Synonyms: Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

Background: The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor, its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response