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ICOS antibody

ICOS Reactivity: Mouse FACS, BR Host: Rat Monoclonal 7E-17G9 unconjugated
Catalog No. ABIN1176983
  • Target See all ICOS Antibodies
    ICOS (Inducible T-Cell Co-Stimulator (ICOS))
    Reactivity
    • 87
    • 23
    • 23
    • 6
    Mouse
    Host
    • 76
    • 17
    • 2
    • 1
    Rat
    Clonality
    • 73
    • 23
    Monoclonal
    Conjugate
    • 39
    • 13
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ICOS antibody is un-conjugated
    Application
    • 64
    • 35
    • 33
    • 24
    • 14
    • 13
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Blocking Reagent (BR)
    Brand
    BD Pharmingen™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
    Immunogen
    Mouse Icos cDNA and ICOS hexahistidine fusion protein
    Clone
    7E-17G9
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product ICOS Primary Antibody
  • Application Notes
    Since this antigen is expressed at only moderate density on activated T lymphocytes, it may be desirable to amplify the staining by using a biotinylated second-step antibody with a bright third-step reagent, such as Streptavidin-PE (Cat. No. 554061). Furthermore, we have found that polyclonal goat anti-rat Ig is weakly reactive with this rat IgG2b mAb, so we recommend the use of biotinylated anti-rat IgG2b mAb RG7/11.1 (Cat. No. 553898) as the second step.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
    Preservative
    Azide free
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • Buonfiglio, Bragardo, Redoglia, Vaschetto, Bottarel, Bonissoni, Bensi, Mezzatesta, Janeway Jr, Dianzani: "The T cell activation molecule H4 and the CD28-like molecule ICOS are identical." in: European journal of immunology, Vol. 30, Issue 12, pp. 3463-7, (2001) (PubMed).

    Chambers: "The expanding world of co-stimulation: the two-signal model revisited." in: Trends in immunology, Vol. 22, Issue 4, pp. 217-23, (2001) (PubMed).

    Dong, Temann, Flavell: "Cutting edge: critical role of inducible costimulator in germinal center reactions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 3659-62, (2001) (PubMed).

    Gonzalo, Delaney, Corcoran, Goodearl, Gutierrez-Ramos, Coyle: "Cutting edge: the related molecules CD28 and inducible costimulator deliver both unique and complementary signals required for optimal T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 1-5, (2001) (PubMed).

    Schwartz: "Immunology. It takes more than two to tango." in: Nature, Vol. 409, Issue 6816, pp. 31-2, (2001) (PubMed).

    Sperling, Bluestone: "ICOS costimulation: It's not just for TH2 cells anymore." in: Nature immunology, Vol. 2, Issue 7, pp. 573-4, (2001) (PubMed).

    Wallin, Liang, Bakardjiev, Sha: "Enhancement of CD8+ T cell responses by ICOS/B7h costimulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 132-9, (2001) (PubMed).

    Mages, Hutloff, Heuck, Büchner, Himmelbauer, Oliveri, Kroczek: "Molecular cloning and characterization of murine ICOS and identification of B7h as ICOS ligand." in: European journal of immunology, Vol. 30, Issue 4, pp. 1040-7, (2000) (PubMed).

    McAdam, Chang, Lumelsky, Greenfield, Boussiotis, Duke-Cohan, Chernova, Malenkovich, Jabs, Kuchroo, Ling, Collins, Sharpe, Freeman: "Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 5035-40, (2000) (PubMed).

  • Target
    ICOS (Inducible T-Cell Co-Stimulator (ICOS))
    Alternative Name
    CD278 (ICOS Products)
    Synonyms
    ICOS antibody, AILIM antibody, CCLP antibody, CRP-1 antibody, H4 antibody, Ly115 antibody, CD278 antibody, CVID1 antibody, Ailim antibody, inducible T-cell costimulator antibody, inducible T cell co-stimulator antibody, inducible T-cell co-stimulator antibody, ICOS antibody, LOC100232284 antibody, Icos antibody
    Background
    The 7E.17G9 antibody reacts with CD278, the Inducible Costimulatory molecule (ICOS), a 47-57 kDa homodimeric glycoprotein of the CD28 family of costimulatory molecules. ICOS is expressed on subpopulations of CD4-CD8- and CD4+CD8- (but not CD4-CD8+ or CD4+CD8+) thymocytes, on some T-cell lines, and on small numbers of peripheral leukocytes. It is upregulated on T lymphocytes following activation via the T-cell receptor. The T-cell activation marker H4 is the same molecule as ICOS. ICOS is a costimulatory receptor, and its ligand on antigen-presenting cells has been called B7RP-1, GL50, B7h, B7-H2, or LICOS. There is considerable evidence that the interaction of ICOS with its ligand is involved in the regulation of many, but not all, T-cell-mediated immune responses.
    Synonyms: ICOS
    Pathways
    Cancer Immune Checkpoints
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