IL-9 antibody

Catalog No. ABIN1176989

Product Details anti-IL-9 Antibody

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This IL-9 antibody is un-conjugated

Application: ELISA, Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-9. Neutralization activity may be measured with a proliferation assay using 500 pg/mL recombinant mouse IL-9 to stimulate MC/9 cells at 5x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-9 for 60 minutes).
50% Neutralization (ND50) at 5 - 25 ng/mL
>95% Neutralization at 50 - 250 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse IL-9 Recombinant Protein

Clone: D9302C12

Isotype: IgG2 kappa

Application Details

Application Notes: ELISA: Biotinylated D9302C12 antibody (Cat. No. 554473) is useful as a detection antibody in sandwich ELISA for measuring mouse IL-9 protein levels. Biotinylated D9302C12 antibody can be paired with the purified D8402E8 antibody (Cat. No. 551218) as the capture antibody, with recombinant mouse IL-9 as the standard.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-9 antibody (ABIN1176989)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Renauld, Kermouni, Vink, Louahed, Van Snick: "Interleukin-9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis." in: Journal of leukocyte biology, Vol. 57, Issue 3, pp. 353-60, (1995) (PubMed).

Nabel, Galli, Dvorak, Dvorak, Cantor: "Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells." in: Nature, Vol. 291, Issue 5813, pp. 332-4, (1981) (PubMed).

Target Details for IL-9

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Alternative Name: IL-9 (IL9 Products)

Synonyms: IL9 antibody, IL-9 antibody, Il-9 antibody, P40 antibody, HP40 antibody, interleukin 9 antibody, IL9 antibody, il9 antibody, Il9 antibody

Background: The D9302C12 monoclonal antibody specifically binds to the multifunctional mouse cytokine, Interleukin-9 (IL-9). IL-9 is a 126 amino acid-long glycoprotein that is produced by various subsets of activated CD4+ T cells. IL-9 acts on target cells by binding to and signaling through the heterodimeric IL-9 receptor (IL-9R) complex that is comprised of transmembrane IL-9 receptor alpha (IL-9Ralpha) and common gamma chain (gammac) subunits. IL-9 can promote the survival, growth, proliferation and/or differentiation of various cell types including thymocytes, T cells, B cells, mast cells, and hematopoietic progenitor cells. IL-9 can augment IL-4-induced IgE and IgG1 production from lipopolysaccharide-primed mouse B cells and induce granzyme and high-affinity IgE receptor gene expression by mouse T helper cell clones and mast cell lines. IL-9 plays an important role in vivo in helminth elimination. The D9302C12 antibody neutralizes mouse IL-9 bioactivity.
Synonyms: IL-9, Interleukin-9, MEA, P40, T-cell growth factor P40, TCGF III

Pathways: JAK-STAT Signaling