VCAM1 antibody

Catalog No. ABIN1176996

Product Details anti-VCAM1 Antibody

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse preadipose cell line PA6

Clone: 429

Isotype: IgG2a kappa

Application Details

Application Notes: For IHC, we recommend the use of purified 429 mAb in our special formulation for immunohistochemistry, Cat. No. 550547.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-VCAM1 antibody (ABIN1176996)

Koni, Joshi, Temann, Olson, Burkly, Flavell: "Conditional vascular cell adhesion molecule 1 deletion in mice: impaired lymphocyte migration to bone marrow." in: The Journal of experimental medicine, Vol. 193, Issue 6, pp. 741-54, (2001) (PubMed).

Buck, Edelman, Buck, Kennedy, Baldwin: "Expression patterns of adhesion receptors in the developing mouse lung: functional implications." in: Cell adhesion and communication, Vol. 4, Issue 2, pp. 69-87, (1997) (PubMed).

Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, (1995) (PubMed).

Kinashi, St Pierre, Springer: "Expression of glycophosphatidylinositol-anchored and -non-anchored isoforms of vascular cell adhesion molecule 1 in murine stromal and endothelial cells." in: Journal of leukocyte biology, Vol. 57, Issue 1, pp. 168-73, (1995) (PubMed).

Baron, Reich, Visintin, Janeway: "The pathogenesis of adoptive murine autoimmune diabetes requires an interaction between alpha 4-integrins and vascular cell adhesion molecule-1." in: The Journal of clinical investigation, Vol. 93, Issue 4, pp. 1700-8, (1994) (PubMed).

Bevilacqua: "Endothelial-leukocyte adhesion molecules." in: Annual review of immunology, Vol. 11, Issue 4, pp. 767-804, (1993) (PubMed).

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: CD106 (VCAM1 Products)

Synonyms: CD106 antibody, INCAM-100 antibody, Vcam-1 antibody, VCAM1B antibody, fi76d06 antibody, wu:fi76d06 antibody, zgc:158875 antibody, VCAM1 antibody, vascular cell adhesion molecule 1 antibody, vascular cell adhesion molecule 1b antibody, VCAM1 antibody, Vcam1 antibody, vcam1b antibody, VCAM-1 antibody

Background: The 429 antibody reacts with both the long (~110 kDa) transmembrane-spanning form and the truncated (~47 kDa) GPI-linked form of vascular cell adhesion molecule-1 (VCAM-1, CD106). CD106 is constitutively expressed on bone marrow stromal cells, myeloid cells, and splenic dendritic cells. Its expression on endothelial cells is upregulated by inflammatory cytokines and in certain pathologic conditions. CD106 expression has also been detected on apoptotic thymocytes, splenocytes, and lymphoid cell lines. VCAM-1 is a counter-receptor for VLA-4 (alpha4beta1 integrin) and LPAM-1 (alpha4beta7 integrin), and the 429 antibody partially blocks VCAM-1-mediated binding functions. Source of the immunogen was mouse preadipose cell line PA6.
Synonyms: VCAM-1

Pathways: Carbohydrate Homeostasis