Western Blotting (WB), ELISA, Neutralization (Neut)
Brand
BD Pharmingen™
Characteristics
Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse TNF. Neutralization activity may be measured using a cytolysis assay for MTT dye conversion with 70 pg/mL recombinant mouse TNF (Cat. No. 554589) and L929 at 2x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse TNF for 60 minutes). 50% Neutralization (ND50) at 30 - 300 ng/mL >95% Neutralization at 2 - 35 myg/mL
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility
0.2 μm filtered
Endotoxin Level
Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative
Azide free
Storage
4 °C
Storage Comment
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
The MP6-XT3 antibody reacts with mouse TNF (TNF-alpha). Cross reactivity with lymphotoxin-alpha (LT-alpha , TNF-beta) has not been determined. Synonyms: Tumor Necrosis Factor