IGF1R antibody (pTyr1131) (Alexa Fluor 647)

Catalog No. ABIN1177067

Product Details anti-IGF1R Antibody (Alexa Fluor 647)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Binding Specificity: pTyr1131

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1R antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human IGF-1 Receptor

Clone: K74-218

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: CD221 (IGF-1 Receptor) (IGF1R Products)

Synonyms: CMT2N antibody, CD221 antibody, IGFIR antibody, IGFR antibody, JTK13 antibody, IGFIRC antibody, IGFR1 antibody, IGF-1R antibody, IGF1R antibody, DKFZp469I0618 antibody, igf-1r antibody, xIGF-1R antibody, xIGFR antibody, xigf1r antibody, A330103N21Rik antibody, D930020L01 antibody, hyft antibody, igf-ir antibody, IGF-IR antibody, IGF-IRb antibody, wu:fb03h06 antibody, IGF-IRa antibody, sb:eu1090 antibody, wu:fi20b10 antibody, alanyl-tRNA synthetase antibody, insulin like growth factor 1 receptor antibody, insulin-like growth factor 1 receptor antibody, insulin-like growth factor 1 antibody, insulin-like growth factor I receptor antibody, insulin like growth factor 1 receptor S homeolog antibody, insulin-like growth factor 1b receptor antibody, insulin-like growth factor 1a receptor antibody, AARS antibody, IGF1R antibody, Igf1r antibody, igf1r antibody, Igf1 antibody, LOC780503 antibody, LOC100136427 antibody, igf1r.S antibody, igf1rb antibody, igf1ra antibody

Background: Insulin-like growth factor-1 (IGF-1) receptor, or CD221, is a receptor tyrosine kinase that has high affinity for IGF-1 and low affinity for insulin and IGF-2. Each transmembrane protein complex is formed from a pair of 1367 amino-acid precursor proteins. After the 30 amino-acid signal sequences are removed, the paired polypeptides are cleaved to derive an a and b chain from each. The two extracellular a chains form the ligand-binding domain, while the transmembrane-and-intracellular b chains carry the kinase activity. Upon stimulation by its ligand, IGF-1 receptor autophosphorylates multiple tyrosine sites in its b chains and phosphorylates other signaling mediators that regulate cell growth, development, and neoplastic transformation. The K74-218 monoclonal antibody recognizes the phosphorylated tyrosine 1131 (pY1131) in the activation loop of IGF-1 receptor’s kinase domain. The orthologous phosphorylation sites in mouse and rat IGF-1 receptor are Y1133 and Y1132, respectively.

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy