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STAT3 antibody (pTyr705) (Pacific Blue)

STAT3 Reactivity: Human, Mouse, Cow, Chimpanzee ICS Host: Mouse Monoclonal 4-P Pacific Blue
Catalog No. ABIN1177198
  • Target See all STAT3 Antibodies
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    Binding Specificity
    • 90
    • 54
    • 27
    • 8
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr705
    Reactivity
    • 277
    • 142
    • 136
    • 38
    • 25
    • 25
    • 22
    • 17
    • 17
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Mouse, Cow, Chimpanzee
    Host
    • 228
    • 58
    • 4
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 196
    • 96
    Monoclonal
    Conjugate
    • 164
    • 23
    • 17
    • 10
    • 9
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    This STAT3 antibody is conjugated to Pacific Blue
    Application
    • 218
    • 110
    • 75
    • 57
    • 51
    • 42
    • 41
    • 41
    • 27
    • 24
    • 15
    • 8
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Intracellular Staining (ICS)
    Brand
    BD Phosflow™
    Purification
    Purified from tissue culture supernatant or ascites by affinity chromatography
    Immunogen
    Phosphorylated Human Stat3 Peptide
    Clone
    4-P
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product STAT3 Primary Antibody
  • Application Notes
    This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells and cell lines (using BD Cytofix™ Fixation Buffer).
    Sample Volume
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody is conjugated to Pacific Blue™ under optimum conditions, and unreacted Pacific Blue™ was removed.
  • Kanai, Konda, Nakajima, Izumi, Kanda, Nanakin, Kubohara, Chiba: "Differentiation-inducing factor-1 (DIF-1) inhibits STAT3 activity involved in gastric cancer cell proliferation via MEK-ERK-dependent pathway." in: Oncogene, Vol. 22, Issue 4, pp. 548-54, (2003) (PubMed).

  • Target
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    Alternative Name
    Stat3 (STAT3 Products)
    Synonyms
    1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody
    Background
    Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat3 is a 92-kDa protein that is activated as a DNA- binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 activation occurs via tyrosine phosphorylation at Y705. Tyrosine phosphorylation in response to cytokine stimulation is generally mediated by JAK1. Upon activation, Stat3 dimerizes, translocates to the nucleus and binds DNA response elements, thereby regulating gene expression. It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to tyrosine phosphorylation, Stat3 is also phosphorylated at S727 via the MAPK pathway. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-gamma induced genes. Thus, phosphorylation of Y705 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity. The 4/P-STAT3 monoclonal antibody recognizes the phosphorylated Y705 of Stat3.
    Synonyms: Acute-phase response factor, APRF
    Pathways
    JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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