WIPF1 antibody (pSer488) (PE)

Catalog No. ABIN1177221

Product Details anti-WIPF1 Antibody (PE)

Target: WIPF1 (WAS/WASL Interacting Protein Family, Member 1 (WIPF1))

Binding Specificity: pSer488

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This WIPF1 antibody is conjugated to PE

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human WIP Peptide

Clone: K32-824

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References for anti-WIPF1 antibody (PE) (ABIN1177221)

Aspenström: "The verprolin family of proteins: regulators of cell morphogenesis and endocytosis." in: FEBS letters, Vol. 579, Issue 24, pp. 5253-9, (2005) (PubMed).

Target Details for WIPF1

Target: WIPF1 (WAS/WASL Interacting Protein Family, Member 1 (WIPF1))

Alternative Name: WIP (WIPF1 Products)

Synonyms: WASPIP antibody, PRPL-2 antibody, WIP antibody, AI115543 antibody, D2Ertd120e antibody, Waspip antibody, Wip antibody, WAS/WASL interacting protein family member 1 antibody, WAS/WASL interacting protein family, member 1 antibody, WIPF1 antibody, Wipf1 antibody

Background: Wiskott-Adrich syndrome protein (WASP)-Interacting Protein (WIP) is a member of the verprolin family of proteins that regulate cytoskeletal organization in a wide variety of cellular activities, including endocytosis, cellular adhesion and migration, mast cell degranulation, and lymphocyte activation. The 503-amino acid WIP protein contains binding sites for actin (globular and filamentous) and other proteins that are involved in the regulation of actin polymerization, such as WASP, N-WASP, profilin, cortactin, Hck, and NCK. As its functions imply, WIP is localized in actin-rich cell structures. The K32-824 monoclonal antibody recognizes the phosphorylated serine 488 (pS488) of human WIP. The orthologous phosphorylation sites in mouse and rat WIP are S478 and S472, respectively.

Pathways: RTK Signaling