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ZAP70 antibody (pTyr292) (Alexa Fluor 647)

ZAP70 Reactivity: Human ICS Host: Mouse Monoclonal J34-602 Alexa Fluor 647
Catalog No. ABIN1177224
  • Target See all ZAP70 Antibodies
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Binding Specificity
    • 40
    • 40
    • 26
    • 20
    • 17
    • 16
    • 14
    • 10
    • 10
    • 9
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr292
    Reactivity
    • 289
    • 125
    • 47
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    Human
    Host
    • 220
    • 65
    • 2
    • 2
    • 1
    Mouse
    Clonality
    • 198
    • 91
    Monoclonal
    Conjugate
    • 145
    • 17
    • 16
    • 14
    • 12
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This ZAP70 antibody is conjugated to Alexa Fluor 647
    Application
    • 200
    • 129
    • 102
    • 67
    • 53
    • 53
    • 47
    • 27
    • 24
    • 22
    • 14
    • 12
    • 6
    • 6
    • 3
    • 2
    • 2
    • 1
    Intracellular Staining (ICS)
    Brand
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Human ZAP70
    Clone
    J34-602
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product ZAP70 Primary Antibody
  • Application Notes
    This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.
    Sample Volume
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
  • Magnan, Di Bartolo, Mura, Boyer, Richelme, Lin, Roure, Gillet, Arrieumerlou, Acuto, Malissen, Malissen: "T cell development and T cell responses in mice with mutations affecting tyrosines 292 or 315 of the ZAP-70 protein tyrosine kinase." in: The Journal of experimental medicine, Vol. 194, Issue 4, pp. 491-505, (2001) (PubMed).

    Rao, Lupher, Ota, Reedquist, Druker, Band: "The linker phosphorylation site Tyr292 mediates the negative regulatory effect of Cbl on ZAP-70 in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 9, pp. 4616-26, (2000) (PubMed).

    Kong, Dalton, Bubeck Wardenburg, Straus, Kurosaki, Chan: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, (1996) (PubMed).

  • Target
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Alternative Name
    Zap70 (ZAP70 Products)
    Synonyms
    SRK antibody, STCD antibody, STD antibody, TZK antibody, ZAP-70 antibody, 70kDa antibody, AI327364 antibody, Srk antibody, mrtle antibody, mur antibody, zap70 antibody, MGC80670 antibody, MGC88962 antibody, ZAP70 antibody, zgc:110383 antibody, zeta chain of T-cell receptor associated protein kinase 70 antibody, putative tyrosine-protein kinase zap-70 antibody, zeta-chain (TCR) associated protein kinase antibody, zeta chain of T cell receptor associated protein kinase 70 antibody, zeta-chain (TCR) associated protein kinase 70kDa antibody, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antibody, zeta chain of T cell receptor associated protein kinase 70kDa antibody, tyrosine-protein kinase ZAP-70 antibody, ZAP70 antibody, Smp_149470 antibody, Zap70 antibody, zap70.L antibody, zap70 antibody, LOC707555 antibody, LOC100342021 antibody, LOC100390922 antibody, LOC100542877 antibody
    Background
    The 70-kDa zeta chain-associated protein (ZAP70) is a Syk-family protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyrosine 493 in the regulatory loop of the PTK domain leading to upregulation of ZAP70 kinase activity. Tyrosine 292 (Y292), in the linker region between the SH2 and PTK domains, is autophosphorylated by the activated PTK domain. By binding with c-Cbl, the phosphorylated Y292 can down-regulate TCR signaling. The J34-602 antibody recognizes ZAP70 phosphorylated at Y292.
    Synonyms: ZAP-70 (pY292)
    Pathways
    TCR Signaling, Ubiquitin Proteasome Pathway
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