ITGB1 antibody

Catalog No. ABIN1177265

Product Details anti-ITGB1 Antibody

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Purified mouse VLA-4

Clone: HM BETA 1-1

Isotype: IgG2 lambda

Application Details

Application Notes: Reported applications include immunoprecipitation and in vitro inhibition (in combination with a mAb to mouse CD11a) of T-cell proliferative responses to anti-CD3e mAb 145-2C11 (Cat. no. 553057) and allogeneic cells. We recommend our immunohistochemistry formulation of purified HM beta1-1 mAb (Cat. No. 550530) for immunohistochemical staining (IHC) of rat tissues. For IHC of mouse tissues, we recommend the use of Purified Rat anti-Mouse CD29 mAb 9EG7 formulated for immunohistochemistry application (Cat. No. 550531).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-ITGB1 antibody (ABIN1177265)

Noto, Kato, Okumura, Yagita: "Identification and functional characterization of mouse CD29 with a mAb." in: International immunology, Vol. 7, Issue 5, pp. 835-42, (1995) (PubMed).

Wadsworth, Chang, Hong, Halvorson, Otto, Coligan: "Expression of a novel integrin beta 1 chain epitope and anti-beta 1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 5, pp. 2125-33, (1995) (PubMed).

Wu, Miyake, Medina, Kincade, Gimble: "Recognition of murine integrin beta 1 by a rat anti-stromal cell monoclonal antibody." in: Hybridoma, Vol. 13, Issue 5, pp. 409-16, (1995) (PubMed).

Mendrick, Kelly: "Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 69, Issue 6, pp. 690-702, (1994) (PubMed).

Jacobsen, Miyake, Kincade, Osmond: "Highly restricted expression of a stromal cell determinant in mouse bone marrow in vivo." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 927-35, (1992) (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29 (ITGB1 Products)

Synonyms: CD29 antibody, FNRB antibody, GPIIA antibody, MDF2 antibody, MSK12 antibody, VLA-BETA antibody, VLAB antibody, 4633401G24Rik antibody, AA409975 antibody, AA960159 antibody, ENSMUSG00000051907 antibody, Fnrb antibody, Gm9863 antibody, gpIIa antibody, cd29 antibody, fnrb antibody, gpiia antibody, itgb1 antibody, mdf2 antibody, msk12 antibody, vla-beta antibody, vlab antibody, itgb1-a antibody, itgb1-b antibody, integrin subunit beta 1 antibody, integrin beta 1 (fibronectin receptor beta) antibody, integrin subunit beta 1 S homeolog antibody, integrin beta 1 antibody, integrin subunit beta 1 L homeolog antibody, ITGB1 antibody, Itgb1 antibody, itgb1.S antibody, LOC100009173 antibody, itgb1.L antibody

Background: The HMbeta1-1 antibody reacts with the 130-kDa integrin beta1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin-alpha chains. With alpha1 through alpha6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with alphav (CD51), it forms alphavbeta1 integrin. It also associates with the integrin alpha7 alpha8, and alpha9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, epithelia, and oocytes. This hamster mAb to a mouse leukocyte antigen has been observed to crossreact with similar populations of rat leukocytes. Source of the immunogen was purified mouse VLA-4 (alpha4beta1, CD49d/CD29).
Synonyms: Itgb1, Integrin beta-1, Fnrb, gpIIa, VLA-4 subunit beta

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome