V-Akt Murine Thymoma Viral Oncogene Homolog 1/2/3 (AKT1/AKT2/AKT3) (C-Term), (AA 460-480) antibody

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C-Term, AA 460-480
(17), (17), (17), (11), (11), (11), (9), (3), (2), (2), (2), (1), (1), (1), (1)
Chicken, Human, Mouse (Murine), Rat (Rattus)
(55), (29), (29), (18), (18), (17), (17), (1)
(46), (8), (1)
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(22), (17), (14), (12), (11), (10), (10), (7), (3)
Pubmed 2 references available
Quantity 0.2 mL
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Catalog No. ABIN117897
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Immunogen Synthetic peptide corresponding to the C-terminus (460-480) of Human, Rat and Mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Sequence C-R-PH-F-P-Q-F-S-Y-S-A-S-G-T-A
Specificity This antiserum reacts with the AKT. Species Reactivity: Tested: Human. Expected from sequence similarity: Rat, Mouse and Chicken.
Purification Serum
Alternative Name AKT1/AKT2/AKT3
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.
Alternate names: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Application Notes ELISA: 1/2,000-1/10,000. Immunoprecipitation. Transfected/Primary cell culture. Western Blot (1/500-1/2,000): Detect a single band of the expected apparent molecularweight. Immunofluorescence using paraformaldehyde-fixed primary cardiomyocyte cultures(1/100-1/1,000). Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections (1/500-1/2,000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by refractometry)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.09% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1/2/3 (AKT1/AKT2/AKT3) (C-Term), (AA 460-480) antibody Immunofluorescence microscopy: Rabbit anti-AKT was used at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red™ conjugated phalloidin.
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1/2/3 (AKT1/AKT2/AKT3) (C-Term), (AA 460-480) antibody (2) Immunoblotting: Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot. Approximately 20 ug of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer. After washing, a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG (R1454HRP) preceded color development using Pierce Chemical's SuperSignal™ substrate.
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1/2/3 (AKT1/AKT2/AKT3) (C-Term), (AA 460-480) antibody (3) Immunohistochemistry. Rabbit anti-AKT was used at a 1:1,000 dilution to detect AKT by immunohistochemistry..,.In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was formalin-fixed and paraffin embedded.
Background publications Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001 (PubMed).

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 (PubMed).

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