PPARA antibody (N-Term)

Catalog No. ABIN117928

Product Details anti-PPARA Antibody

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Binding Specificity: AA 1-18, N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against Mouse PPAR alpha protein. Reactivity against PPAR alpha from other species has not been tested. No reactivity is expected against other subtypes of PPAR.

Cross-Reactivity (Details): Species reactivity (expected):A BLAST analysis was used to suggest reactivity with this protein from Mouse, Rat, Bovine, Dog, Golden Hamster and Boar sources based on 100 % homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from Human, Chimpanzee and Rhesus Monkey may also occur as this sequence shows 88 % homology (16/18 identities) with the protein from these sources.
Species reactivity (tested):Mouse.

Purification: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.

Immunogen: Synthetic peptide corresponding to amino acids 1 to 18 of Human PPAR alpha.

Application Details

Application Notes: Suitable for use in ELISA (1/10,000-1/100,000) and Western blotting (1/500-1/2,000). Reactivity in other immunoassays is unknown. Recommended Dilutions: This product has been assayed by ELISA against 0.1 μg of theimmunizing peptide. A 1/8,000 to 1/32,000 dilution of the antibody is recommended for

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPAR-alpha (PPARA Products)

Synonyms: NR1C1 antibody, PPAR antibody, PPARalpha antibody, hPPAR antibody, LOC100136415 antibody, PPARALPHA antibody, nr1c1 antibody, xPPARalpha antibody, xppara antibody, 4933429D07Rik antibody, AW742785 antibody, Nr1c1 antibody, PPAR-alpha antibody, Ppar antibody, peroxisome proliferator activated receptor alpha antibody, peroxisome proliferator-activated receptor alpha antibody, peroxisome proliferator activated receptor alpha L homeolog antibody, PPARA antibody, LOC100136415 antibody, ppara.L antibody, Ppara antibody, ppara antibody

Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPARgamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.Synonyms: NR1C1, Nuclear receptor subfamily 1 group C member 1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha

Gene ID: 5465

NCBI Accession: NP_001001928

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C