PPARG antibody

Catalog No. ABIN117929

Product Details anti-PPARG Antibody

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: Peptide sequence: M-G-E-D-K-I-K-F-K-H-I-T-P-L Protein Sequence: Human PPAR gamma isoform 1, 475 aa, predicted MW 54.4 kDa 1 mvdtempfwp tnfgissvdl svmedhshsf dikpfttvdf ssistphyed ipftrtdpvv 61 adykydlklq eyqsaikvep asppyysekt qlynkpheep snslmaiecr vcgdkasgfh 121 ygvhacegck gffrrtirlk liydrcdlnc rihkksrnkc qycrfqkcla vgmshnairf 181 grmpqaekek llaeissdid qlnpesadlr alakhlydsy iksfpltkak arailtgktt 241 dkspfviydm nslmmgedki kfkhitplqe qskevairif qgcqfrsvea vqeiteyaks 301 ipgfvnldln dqvtllkygv heiiytmlas lmnkdgvlis egqgfmtref lkslrkpfgd 361 fmepkfefav kfnaleldds dlaifiavii lsgdrpglln vkpiediqdn llqalelqlk 421 lnhpessqlf akllqkmtdl rqivtehvql lqvikktetd mslhpllqei ykdly Protein Sequence: Human PPAR gamma isoform 2, 505 aa, predicted MW 57.6 kDa 1 mgetlgdspi dpesdsftdt lsanisqemt mvdtempfwp tnfgissvdl svmedhshsf 61 dikpfttvdf ssistphyed ipftrtdpvv adykydlklq eyqsaikvep asppyysekt 121 qlynkpheep snslmaiecr vcgdkasgfh ygvhacegck gffrrtirlk liydrcdlnc 181 rihkksrnkc qycrfqkcla vgmshnairf grmpqaekek llaeissdid qlnpesadlr 241 alakhlydsy iksfpltkak arailtgktt dkspfviydm nslmmgedki kfkhitplqe 301 qskevairif qgcqfrsvea vqeiteyaks ipgfvnldln dqvtllkygv heiiytmlas 361 lmnkdgvlis egqgfmtref lkslrkpfgd fmepkfefav kfnaleldds dlaifiavii 421 lsgdrpglln vkpiediqdn llqalelqlk lnhpessqlf akllqkmtdl rqivtehvql 481 lqvikktetd mslhpllqei ykdly

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This product is expected to react with human (100 % identity with immunogen) due to sequence homology. Reactivity against PPAR gamma 1 or from other species has not been tested.

Purification: Immunoaffinity chromatography.

Immunogen: Antibody R1490P was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to human PPAR gamma 1 and 2.

Application Details

Application Notes: This product has been assayed by ELISA against 0.1 g of the immunizing peptide. A1: 7,000 to 1: 33,000 dilution of the antibody is recommended for this assay. Reactivity in other immunoassays is unknown.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, and 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPAR-gamma (PPARG Products)

Synonyms: PPAR gamma antibody, PPARg2 antibody, PPARG antibody, Nr1c3 antibody, PPAR-gamma antibody, PPAR-gamma2 antibody, PPARgamma antibody, PPARgamma2 antibody, NR1C3 antibody, CIMT1 antibody, GLM1 antibody, PPARG1 antibody, PPARG2 antibody, xPPAR-gamma antibody, PPAR-GAMMA antibody, PPARGAMMA antibody, peroxisome proliferator-activated receptor gamma antibody, peroxisome proliferator activated receptor gamma antibody, peroxisome proliferator activated receptor gamma L homeolog antibody, PPARG antibody, Pparg antibody, pparg.L antibody

Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and antidiabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Gene ID: 5468

NCBI Accession: NP_005028

UniProt: P37231

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation