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Signal Transducer and Activator of Transcription 4 (STAT4) (AA 730-749) antibody

Details for Product No. ABIN117931
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Antigen
Synonyms SLEB11
Epitope
AA 730-749
(42), (11), (8), (7), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(162), (87), (76), (27), (26), (25), (13)
Host
Rabbit
(134), (30), (6), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(127), (63), (48), (39), (34), (30), (19), (5), (5), (2), (2), (2), (2)
Pubmed 8 references available
Quantity 0.1 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN117931
390.50 $
Plus shipping costs $45.00

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 730-749 of Mouse STAT -4. Swiss Prot accession number P42228.
Sequence Peptide sequence: R-E-N-L-S-P-T-T-I-E-T-A-M-N-S-P-Y-S-A-E Protein sequence: Mouse STAT4 protein is composed of 749 amino acids (85.9 kDa) 1 msqwnqvqql eikfleqvdq fyddnfpmei rhllaqwiet dwevasnne tmatillqnl 61 liqldeqlgr vskeknllli hnlkrirkvl qgkfhgnpmh vavvisnclr eerrilaaan 121 mpiqgpleks lqsssvserq rnvehkvsai knsvqmteqd tkyledlqde fdyryktiqt 181 mdqgdknsil vnqevltllq emlnsldfkr kealskmtqi vnetdllmns mlleelqdwk 241 krqqiacigg plhngldqlq ncftllaesl fqlrqqlekl qeqstkmtye gdpipaqrah 301 lleratfliy nlfknsfvve qpcmpthpq rpmvlktliq tvklrllik lpelnyqvkv 361 kasidknvst lsnrrfvlcg thvkamssee ssngslsvef rhlqpkemkc stgskgnegc 421 hmvteelhsi tfetqiclyg ltinletssl pvvmisnvsq lpnawasiiw ynvstndsqn 481 lvffnnppsv tlgqllevms wqfssyvgrg lnseqlnmla ekltvqsnyn dghltwakfc 541 kehlpgktft fwtwleaild likkhilplw idgyimgfvs kekerlllkd kmpgtfllrf 601 seshlggitf twvdqsenge vrfhsvepyn kgrlsalafa dilrdykvim aenipenplk 661 ylypdipkdk afgkhyssqp cevsrpterg dkgyvpsvfi pistirsdst epqspsdllp 721 mspsayavlr enlspttiet amnspysae
Specificity This antibody was prepared from whole rabbit antiserum by delipidation and defibrination. Reactivity occurs against mouse STAT4 protein. Cross reactivity with closely related STAT family members has not been determined. Cross reactivity is expected against human STAT4 protein based on a 95% homology of the amino acid sequence of the immunogen between human and mouse. A single amino acid change is reported (from N to K). Cross reactivity is expected against rat STAT4.
Purification Delipidation and defibrination.
Alternative Name STAT4
Background Signal transducer and activator of transcription 4 (STAT4) carries out a dual function of signal transduction and activation of transcription. STAT4 forms a homodimer or a heterodimer with related transcription factor STAT family members. STAT4 has a cytoplasmic localization and is translocated into the nucleus in response to activation by JAK, which phosphorylates STAT4 at a tyrosine residue. Various ligands, including interferons and growth factors, induce activation of the JAK kinases, which induce STAT activation. IL-12 has also been identified as an activator of STAT4. Serine phosphorylation is also required for maximal transcriptional activity. The highest level of STAT4 expression is observed in testis and in myeloid cells.
Alternate names: Signal transducer and activator of transcription 4
Gene ID 6775
NCBI Accession NP_003142.1
UniProt Q14765
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Signaling, Receptors, Inflammation
Application Notes This antibody has been tested for use in ELISA, Western blotting and Immunoprecipitation. Reactivity in other immunoassays is unknown. A HeLa whole cell lysate or mouse testislysate are suitable for use as a positive control. Recommended Dilutions: This product was assayed by western blotting afterImmunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1: 5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 ul for Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
Expiry Date 12 months
Supplier Images
anti-Signal Transducer and Activator of Transcription 4 (STAT4) (AA 730-749) antibody ",Western blot following immunoprecipitation of STAT4 using ROCKLAND Immunochemical's Rabbit-anti-STAT4 antibody. Lane 2 shows the detection of a single band corresponding to mouse STAT4. Molecular weight markers (not shown) confirm a MW of ~ 85 kDa. For IP use approximately 5 ul of antiserum. A 1:5,000 dilution of the antiserum was used for western blotting. Detection occurs using a 1:2,000 dilution of HRP Goat-a-Rabbit IgG (ABIN117865) with visualization via ECL. Film exposure approximately 45",",. Other detection systems will yield similar results.",
Background publications Zhong, Wen, Darnell: "Stat3 and Stat4: members of the family of signal transducers and activators of transcription." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 11, pp. 4806-10, 1994 (PubMed).

Yamamoto, Quelle, Thierfelder et al.: "Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation." in: Molecular and cellular biology, Vol. 14, Issue 7, pp. 4342-9, 1994 (PubMed).

Matikainen, Sareneva, Ronni et al.: "Interferon-alpha activates multiple STAT proteins and upregulates proliferation-associated IL-2Ralpha, c-myc, and pim-1 genes in human T cells." in: Blood, Vol. 93, Issue 6, pp. 1980-91, 1999 (PubMed).

Schindler, Lutz, Röllinghoff et al.: "The production of IFN-gamma by IL-12/IL-18-activated macrophages requires STAT4 signaling and is inhibited by IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3075-82, 2001 (PubMed).

Poltorak, Merlin, Nielsen et al.: "A point mutation in the IL-12R beta 2 gene underlies the IL-12 unresponsiveness of Lps-defective C57BL/10ScCr mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 4, pp. 2106-11, 2001 (PubMed).

Freudenberg, Merlin, Kalis et al.: "Cutting edge: a murine, IL-12-independent pathway of IFN-gamma induction by gram-negative bacteria based on STAT4 activation by Type I IFN and IL-18 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1665-8, 2002 (PubMed).

OSullivan, Chang, Yu et al.: "STAT4 is required for interleukin-12-induced chromatin remodeling of the CD25 locus." in: The Journal of biological chemistry, Vol. 279, Issue 8, pp. 7339-45, 2004 (PubMed).

General Baum, Trabanco, Montalban et al.: "Synthesis and reactions of aminoporphyrazines with annulated five- and seven-membered rings." in: The Journal of organic chemistry, Vol. 68, Issue 5, pp. 1665-70, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN117931
390.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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