ANAPC2 antibody (C-Term)

Catalog No. ABIN117942

Product Details anti-ANAPC2 Antibody

Target: ANAPC2 (Anaphase Promoting Complex Subunit 2 (ANAPC2))

Binding Specificity: AA 810-822, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Sequence: Human APC2 (822 aa 93.8 kDa): 1 maaavvvaeg dsdsrpgqel lvawntvstg lvppaalglv ssrtsgavpp keeelraave 61 vlrghglhsv leewfvevlq ndlqanispe fwnaisqcen sadepqclll lldafglles 121 rldpylrsle llekwtrlgl lmgtgaqglr eevhtmlrgv lffstprtfq emiqrlygcf 181 lrvymqskrk geggtdpele geldsryarr ryyrllqspl cagcssdkqq cwcrqaleqf 241 hqlsqvlhrl sllervsaea vtttlhqvtr ermedrcrge yersflrefh kwiervvgwl 301 gkvflqdgpa rpaspeagnt lrrwrchvqr ffyriyaslr ieelfsivrd fpdsrpaied 361 lkyclertdq rqqllvslka aletrllhpg vntcdiitly isaikalrvl dpsmvileva 421 cepirrylrt redtvrqiva gltgdsdgtg dlavelsktd pasletgqds eddsgepedw 481 vpdpvdadpg kssskrrssd iisllvsiyg skdlfineyr slladrllhq fsfspereir 541 nvellklrfg eapmhfcevm lkdmadsrri nanireedek rpaeeqppfg vyavilssef 601 wppfkdekle vpediraale ayckkyeqlk amrtlswkht lglvtmdvel adrtlsvavt 661 pvqavillyf qdqaswtlee lskavkmpva llrrrmsvwl qqgvlreepp gtfsvieeer 721 pqdrdnmvli dsddesdsgm asqadqkeee lllfwtyiqa mltnleslsl driynmlrmf 781 vvtgpalaei dlqelqgylq kkvrdqqlvy sagvyrlpkn cs

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This antibody reacts with human APC2. Cross reactivity may also occur with APC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.

Purification: Delipidation and defibrination.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 810-822 of Human APC2 (C- terminal) coupled to KLH.

Application Details

Application Notes: Western blot (1: 500-1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others. Coimmunoprecipitation of related proteins (APC11) does occur. A 93.8 kDa bandcorresponding to human APC2 is detected. Most cell lines or tissues expressing APC2 canbe used as a positive control. ELISA (1: 2,000-1: 10,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ANAPC2 antibody (ABIN117942)

Wang, Yu, Xiang, Fan, He, Guo, Feng, Deng: "Global transcriptional profiles of Staphylococcus aureus treated with berberine chloride." in: FEMS microbiology letters, Vol. 279, Issue 2, pp. 217-25, (2008) (PubMed).

Target Details for ANAPC2

Target: ANAPC2 (Anaphase Promoting Complex Subunit 2 (ANAPC2))

Alternative Name: APC2 / ANAPC2 (ANAPC2 Products)

Synonyms: anapc2 antibody, ANAPC2 antibody, DDBDRAFT_0203816 antibody, DDBDRAFT_0234264 antibody, DDB_0203816 antibody, DDB_0234264 antibody, si:ch73-203i15.5 antibody, APC2 antibody, RP11-350O14.5 antibody, 9230107K09Rik antibody, AL024279 antibody, Apc2 antibody, Emi4 antibody, Imi4 antibody, mKIAA1406 antibody, F28I8.30 antibody, F28I8_30 antibody, anaphase-promoting complex/cyclosome 2 antibody, anaphase promoting complex subunit 2 antibody, anaphase promoting complex subunit 2 L homeolog antibody, anaphase-promoting complex/cyclosome 2 antibody, Anaphase-promoting complex subunit 2 antibody, ANAPC2 antibody, anapc2.L antibody, anapc2 antibody, Anapc2 antibody, APC2 antibody, apc-2 antibody

Background: APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, and ANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/cyclosome protein complex promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at least eleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin family member that interacts through the cullin domain with ANAPC11 and UBCH10.Synonyms: Anaphase-promoting complex subunit 2, Cyclosome subunit 2, KIAA1406

Gene ID: 29882

NCBI Accession: NP_037498

UniProt: Q9UJX6

Pathways: Regulation of Cell Size