CAND1 antibody (N-Term)

Catalog No. ABIN117944

Product Details anti-CAND1 Antibody

Target: CAND1 (Cullin-Associated and Neddylation-Dissociated 1 (CAND1))

Binding Specificity: AA 11-24, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAND1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: Human CAND1 (TIP120A) (1230 aa 136.4 kDa): 1 masasyhisn llekmtssdk dfrfmatndl mtelqkdsik ldddserkvv kmilklledk 61 ngevqnlavk clgplvskvk eyqvetivdt lctnmlsdke qlrdissigl ktvigelppa 121 ssgsalaanv ckkitgrlts aiakqedvsv qlealdimad mlsrqggllv nfhpsiltcl 181 lpqltsprla vrkrtiialg hlvmscgniv fvdliehlls elskndsmst trtyiqciaa 241 isrqaghrig eylekiiplv vkfcnvddde lreyciqafe sfvrrcpkev yphvstiini -- 901 sqpkrqylll hslkeiissa svvglkpyve niwalllkhc ecaeegtrnv vaeclgkltl 961 idpetllprl kgylisgssy arssvvtavk ftisdhpqpi dpllkncigd flktledpdl 1021 nvrrvalvtf nsaahnkpsl irdlldtvlp hlynetkvrk elirevemgp fkhtvddgld 1081 irkaafecmy tlldscldrl difeflnhve dglkdhydik mltflmlvrl stlcpsavlq 1141 rldrlveplr atcttkvkan svkqefekqd elkrsamrav aalltipeae ksplmsefqs 1201 qissnpelaa ifesiqkdss stnlesmdts

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This antibody reacts with human, rat and mouse CAND1/TIP120A. Cross reactivity does occur with human, rat and mouse CAND2/TIP120B. Cross reactivity with CAND1 from other sources is not known.

Purification: Delipidation and defibrination.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 11-24 of Human CAND1/TIP120A (N- terminal) coupled to KLH.

Application Details

Application Notes: Western blot (1: 500-1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CAND1 antibody (ABIN117944)

Concejero, Chen, Wang, Wang, Lin, Liu, Yang, Yong, Lin, Jawan, Huang, Cheng, Eng: "Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan." in: Transplantation, Vol. 85, Issue 3, pp. 398-406, (2008) (PubMed).

Kim, Min, Lee, Kim, Oh, Jeon, Lee, Im, Chung, Kim, Kim, Bang, Sung, Kim, Heo: "High tumour islet macrophage infiltration correlates with improved patient survival but not with EGFR mutations, gene copy number or protein expression in resected nonsmall cell lung cancer." in: British journal of cancer, (2008) (PubMed).

Target Details for CAND1

Target: CAND1 (Cullin-Associated and Neddylation-Dissociated 1 (CAND1))

Alternative Name: CAND1 (CAND1 Products)

Synonyms: TIP120 antibody, TIP120A antibody, ATCAND1 antibody, CULLIN-ASSOCIATED AND NEDDYLATION DISSOCIATED 1 antibody, ETA2 antibody, HEMIVENATA antibody, HVE antibody, T8K22.14 antibody, T8K22_14 antibody, cullin-associated and neddylation dissociated antibody, Tip120 antibody, Tip120A antibody, fb78a05 antibody, wu:fb78a05 antibody, zgc:55729 antibody, tip120 antibody, tip120a antibody, cand2 antibody, 2310038O07Rik antibody, 6330512O03Rik antibody, AI195005 antibody, AI846556 antibody, D10Ertd516e antibody, Tp120a antibody, mKIAA0829 antibody, cullin associated and neddylation dissociated 1 antibody, cullin-associated and neddylation dissociated antibody, cullin-associated and neddylation-dissociated 1 antibody, cullin-associated and neddylation-dissociated 1 L homeolog antibody, cullin-associated NEDD8-dissociated protein 1 antibody, cullin associated and neddylation disassociated 1 antibody, CAND1 antibody, Cand1 antibody, cand1 antibody, cand1.L antibody, LOC100159824 antibody, LOC100177977 antibody

Background: CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.Synonyms: Cullin-associated NEDD8-dissociated protein 1, Cullin-associated and neddylation-dissociated protein 1, KIAA0829, TBP-interacting protein TIP120A, TBP-interacting protein of 120 kDa A, TIP120, TIP120A, p120 CAND1

Gene ID: 55832

NCBI Accession: NP_060918

UniProt: Q86VP6

Pathways: Regulation of Actin Filament Polymerization