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CAND1 antibody (Cullin-Associated and Neddylation-Dissociated 1) (C-Term)

Details for Product anti-CAND1 Antibody No. ABIN117945, Supplier: Log in to see
Antigen
  • 2310038O07Rik
  • 6330512O03Rik
  • AI195005
  • AI846556
  • ATCAND1
  • cand2
  • cullin-associated and neddylation dissociated
  • CULLIN-ASSOCIATED AND NEDDYLATION DISSOCIATED 1
  • D10Ertd516e
  • ETA2
  • fb78a05
  • HEMIVENATA
  • HVE
  • mKIAA0829
  • T8K22.14
  • T8K22_14
  • Tip120
  • TIP120
  • tip120
  • tip120a
  • TIP120A
  • Tip120A
  • Tp120a
  • wu:fb78a05
  • zgc:55729
Epitope
C-Term, AA 1130-1143
13
6
6
5
4
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
33
8
6
3
2
2
2
1
Host
Rabbit
20
13
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
31
18
10
9
8
6
2
Supplier
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Immunogen This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.
Sequence Human CAND2 (TIP120B) (1143 aa 125.4 kDa): 1 mstaafhiss llekmtssdk dfrfmatsdl mselqkdsiq ldedserkvv kmllrlledk 61 ngevqnlavk wlgvplgafh asllhcllpq lssprlavrk ravgalghla aacstdlfve 121 ladhlldrlp gprvptspta irtliqclgs vgrqaghrlg ahldrlvplv edfcnlddde 181 lresclqafe aflrkcpkem gphvpnvtsl clqyikhdpn ynydsdedee qmetedsefs 241 eqesedeysd dddmswkvrr aaakciaali ssrpdllpdf hctlapvlir rfkereenvk --- 901 vkflisdqph pidpllksfi gefmeslqdp dlnvrratla ffnsavhnkp slvrdllddi 961 lpllyqetki rrdlirevem gpfkhtvddg ldvrkaafec mysllesclg qldiceflnh 1021 vedglkdhyd irmltfimva rlatlcpapv lqrvdrliep lratctakvk agsvkqefek 1081 qdelkrsamr avaalltipe vgkspimadf ssqirsnpel aalfesiqkd sasapstdsm 1141 els
Specificity This antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. It reacts with human, rat and mouse CAND2/TIP120B. Cross reactivity does occur with human, rat and mouse CAND1/TIP120A. Cross reactivity with CAND2 from other sources is not known.
Purification Delipidation and defibrination.
Alternative Name CAND1 (CAND1 Antibody Abstract)
Background CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60 % identical in amino acid sequence to TIP120A.Synonyms: Cullin-associated NEDD8-dissociated protein 1, Cullin-associated and neddylation-dissociated protein 1, KIAA0829, TBP-interacting protein TIP120A, TBP-interacting protein of 120 kDa A, TIP120, TIP120A, p120 CAND1
Gene ID 55832
NCBI Accession NP_060918
UniProt Q86VP6
Research Area Apoptosis/Necrosis, Proteasome
Application Notes Western blot (1: 500-1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from transfected cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been tested. A 125.4 kDa bandcorresponding to human CAND2 is detected. CAND2 is specifically expressed in muscle
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-CAND1 antibody (Cullin-Associated and Neddylation-Dissociated 1) (C-Term) (ABIN117945) Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifier...
Product cited in: Concejero, Chen, Wang, Wang, Lin, Liu, Yang, Yong, Lin, Jawan, Huang, Cheng, Eng: "Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan." in: Transplantation, Vol. 85, Issue 3, pp. 398-406, 2008

Background publications Feng, Shen, Sullivan, Rubio, Xiong, Sun, Deng: "Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation." in: The Plant cell, Vol. 16, Issue 7, pp. 1870-82, 2004

Cheng, Dai, Zhao: "AtCAND1, a HEAT-repeat protein that participates in auxin signaling in Arabidopsis." in: Plant physiology, Vol. 135, Issue 2, pp. 1020-6, 2004

Oshikawa, Matsumoto, Yada, Kamura, Hatakeyama, Nakayama: "Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1." in: Biochemical and biophysical research communications, Vol. 303, Issue 4, pp. 1209-16, 2003

Liu, Furukawa, Matsumoto, Xiong: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002

Zheng, Yang, Harrell, Ryzhikov, Shim, Lykke-Andersen, Wei, Sun, Kobayashi, Zhang: "CAND1 binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex." in: Molecular cell, Vol. 10, Issue 6, pp. 1519-26, 2002

Makino, Yogosawa, Kayukawa, Coin, Egly, Wang, Roeder, Yamamoto, Muramatsu, Tamura: "TATA-Binding protein-interacting protein 120, TIP120, stimulates three classes of eukaryotic transcription via a unique mechanism." in: Molecular and cellular biology, Vol. 19, Issue 12, pp. 7951-60, 2000

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000

Makino, Yoshida, Yogosawa, Tanaka, Muramatsu, Tamura: "Multiple mammalian proteasomal ATPases, but not proteasome itself, are associated with TATA-binding protein and a novel transcriptional activator, TIP120." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 4, Issue 9, pp. 529-39, 1999