This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.
Purification
Delipidation and defibrination.
Immunogen
Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.
Western blot: This antibody reacts with human CDC20 (fizzy) (1: 500 - 1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa bandcorresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressingCDC20 can be used as a positive control.
Restrictions
For Research Use only
Format
Liquid
Concentration
85 mg/mL (by Refractometry)
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
Target
CDC20
(Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.Synonyms: Cell division cycle protein 20 homolog, p55CDC