You are viewing an incomplete version of our website. Please click to reload the website as full version.

CDC20 antibody (Cell Division Cycle 20 Homolog (S. Cerevisiae)) (C-Term)

Details for Product anti-CDC20 Antibody No. ABIN117946, Supplier: Log in to see
Antigen
  • 2310042N09Rik
  • bA276H19.3
  • ba276h19.3
  • C87100
  • CDC20A
  • cdc20a
  • fizzy1
  • p55cdc
  • p55CDC
  • X-FZY
Epitope
C-Term, AA 486-499
23
5
3
3
3
2
2
2
1
1
1
1
Reactivity
Human
77
21
20
4
3
2
2
2
2
Host
Rabbit
66
15
Clonality
Polyclonal
Conjugate
This CDC20 antibody is un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
61
34
23
22
15
10
9
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.
Sequence Human CDC20 (fizzy) (499 aa 54.7 kDa): 1 maqfafesdl hsllqldapi pnapparwqr kakeaagpap spmraanrsh sagrtpgrtp 61 gkssskvqtt pskpggdryi phrsaaqmev asfllskenq sensqtptkk ehqkawalnl 21 ngfdveeaki lrlsgkpqna pegyqnrlkv lysqkatpgs srktcryips lpdrildape 181 irndyylnlv dwssgnvlav aldnsvylws assgdilqll qmeqpgeyis svawikegny 241 lavgtssaev qlwdvqqqkr lrnmtshsar vgslswnsyi lssgsrsghi hhhdvrvaeh 301 hvatlsghsq evcglrwapd grhlasggnd nlvnvwpsap geggwvplqt ftqhqgavka 361 vawcpwqsnv latgggtsdr hiriwnvcsg aclsavdahs qvcsilwsph ykelisghgf 421 aqnqlviwky ptmakvaelk ghtsrvlslt mspdgatvas aaadetlrlw rcfeldparr 481 rerekasaak sslihqgir
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.
Purification Delipidation and defibrination.
Alternative Name CDC20 (CDC20 Antibody Abstract)
Background CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.Synonyms: Cell division cycle protein 20 homolog, p55CDC
Gene ID 991
NCBI Accession NP_001246
UniProt Q12834
Research Area Cell Cycle
Application Notes Western blot: This antibody reacts with human CDC20 (fizzy) (1: 500 - 1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa bandcorresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressingCDC20 can be used as a positive control.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-CDC20 antibody (Cell Division Cycle 20 Homolog (S. Cerevisiae)) (C-Term) (ABIN117946) Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifier...
Background publications Song, Song, Ayad, Chang, Lee, Hong, Lee, Choi, Kim, Kim, Kim, Choi, Kirschner, Lim: "The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex." in: Nature cell biology, Vol. 6, Issue 2, pp. 129-37, 2004

Zur, Brandeis: "Timing of APC/C substrate degradation is determined by fzy/fzr specificity of destruction boxes." in: The EMBO journal, Vol. 21, Issue 17, pp. 4500-10, 2002

Asadi, Jörgensen, Jacobsson: "Elovl1 and p55Cdc genes are localized in a tail-to-tail array and are co-expressed in proliferating cells." in: The Journal of biological chemistry, Vol. 277, Issue 21, pp. 18494-500, 2002

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000

Cahill, da Costa, Carson-Walter, Kinzler, Vogelstein, Lengauer: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999

Weinstein, Karim, Geschwind, Nelson, Krumm, Sakamoto: "Genomic organization, 5' flanking enhancer region, and chromosomal assignment of the cell cycle gene, p55Cdc." in: Molecular genetics and metabolism, Vol. 64, Issue 1, pp. 52-7, 1998

Weinstein: "Cell cycle-regulated expression, phosphorylation, and degradation of p55Cdc. A mammalian homolog of CDC20/Fizzy/slp1." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28501-11, 1997

Weinstein, Jacobsen, Hsu-Chen, Wu, Baum: "A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3350-63, 1994