Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Cullin 1 antibody (C-Term)

CUL1 Reactivity: Human, Mouse WB, IP, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN117947
  • Target See all Cullin 1 (CUL1) Antibodies
    Cullin 1 (CUL1)
    Binding Specificity
    • 15
    • 15
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 742-752, C-Term
    Reactivity
    • 78
    • 33
    • 28
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse
    Host
    • 77
    • 1
    Rabbit
    Clonality
    • 66
    • 12
    Polyclonal
    Conjugate
    • 40
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Cullin 1 antibody is un-conjugated
    Application
    • 44
    • 27
    • 15
    • 15
    • 14
    • 13
    • 12
    • 8
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Sequence
    Human Cullin 1 (776 aa 89.6 kDa): 1 msstrsqnph glkqigldqi wddlragiqq vytrqsmaks rymelythvy nyctsvhqsn 61 qargagvpps kskkgqtpgg aqfvglelyk rlkeflknyl tnllkdgedl mdesvlkfyt 121 qqwedyrfss kvlngicayl nrhwvrrecd egrkgiyeiy slalvtwrdc lfrplnkqvt 181 navlklieke rngetintrl isgvvqsyve lglneddafa kgptltvyke sfesqfladt 241 erfytreste flqqnpvtey mkkaearlle eqrrvqvylh estqdelark ceqvliekhl 301 eifhtefqnl ldadknedlg rmynlvsriq dglgelkkll ethihnqgla aiekcgeaal 361 ndpkmyvqtv ldvhkkynal vmsafnndag fvaaldkacg rfinnnavtk maqssskspe 421 llarycdsll kkssknpeea eledtlnqvm vvfkyiedkd vfqkfyakml akrlvhqnsa 481 sddaeasmis klkqacgfey tsklqrmfqd igvskdlneq fkkhltnsep ldldfsiqvl 541 ssgswpfqqs ctfalpsele rsyqrftafy asrhsgrklt wlyqlskgel vtncfknryt 601 lqastfqmai llqynteday tvqqltdstq ikmdilaqvl qillkskllv ledenanvde 661 velkpdtlik lylgyknkkl rvninvpmkt eqkqeqetth knieedrkll iqaaivrimk 721 mrkvlkhqql lgevltqlss rfkprvpvik kcidilieke ylervdgekd tysyla
    Specificity
    This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology.
    Purification
    Delipidation and defibrination.
    Immunogen
    Synthetic peptide corresponding to amino acids 742-752 of Human Cul1 (C-terminus) coupled to KLH.
    Top Product
    Discover our top product CUL1 Primary Antibody
  • Application Notes
    Western blot: This antibody reacts with human Cul1 (1/500-1/1,000, An 89.6 kDa bandcorresponding to human Cul1 is detected). Most cell lines expressing Cul1 can be used as apositive control. Immunoprecipitation: The antibody immunoprecipitates in vitro translated product andprotein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, butrather use 0.1 % SDS). ELISA (1/2,000-1/10,000).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    85.0 mg/mL (by Refractometry)
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or at -20 °C for longer.
  • Target
    Cullin 1 (CUL1)
    Alternative Name
    Cullin 1 (CUL1 Products)
    Synonyms
    LOC100282292 antibody, Cullin-1 antibody, Cul1 antibody, zgc:55747 antibody, zgc:77500 antibody, wu:fc13a10 antibody, cullin-1 antibody, ATCUL1 antibody, AUXIN RESISTANT 6 antibody, AXR6 antibody, T10P11.26 antibody, T10P11_26 antibody, cullin 1 antibody, cul1 antibody, wu:fi43h07 antibody, wu:fj43a08 antibody, zgc:111899 antibody, zgc:55483 antibody, Cul-1 antibody, cullin 1 antibody, cullin-1 antibody, Cullin 1 antibody, Cullin-1 antibody, cullin 1a antibody, cullin 1 S homeolog antibody, cullin 1b antibody, CUL1 antibody, LOC100282292 antibody, cul1 antibody, ATEG_00491 antibody, EDI_072700 antibody, EDI_264400 antibody, SJAG_02591 antibody, BDBG_03785 antibody, Cul1 antibody, cul1a antibody, cul1.S antibody, cul-1 antibody, cul1b antibody
    Background
    Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 1 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul1 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul1 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul1 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.Synonyms: CUL-1, CUL1, Cullin-1
    Gene ID
    8454
    NCBI Accession
    NP_003583
    UniProt
    Q13616
    Pathways
    Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size
You are here:
Support