Cullin 1 (CUL1) (C-Term), (AA 742-752) antibody

Details for Product No. ABIN117947
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Synonyms LOC100282292, Cul1, zgc:55747, zgc:77500, wu:fc13a10, CUL1, cullin-1, Cullin-1, ATCUL1, AUXIN RESISTANT 6, AXR6, T10P11.26, T10P11_26, cullin 1, cul1, wu:fi43h07, wu:fj43a08, zgc:111899, zgc:55483
C-Term, AA 742-752
(49), (15), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(108), (40), (24), (2), (2), (2), (1), (1), (1), (1), (1)
(102), (6), (1)
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(91), (41), (37), (26), (21), (21), (10), (6), (5), (3), (2), (2), (1), (1), (1)
Pubmed 8 references available
Quantity 0.1 mL
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Catalog No. ABIN117947
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Immunogen Synthetic peptide corresponding to amino acids 742-752 of Human Cul1 (C-terminus) coupled to KLH.
Sequence Human Cullin 1 (776 aa 89.6 kDa): 1 msstrsqnph glkqigldqi wddlragiqq vytrqsmaks rymelythvy nyctsvhqsn 61 qargagvpps kskkgqtpgg aqfvglelyk rlkeflknyl tnllkdgedl mdesvlkfyt 121 qqwedyrfss kvlngicayl nrhwvrrecd egrkgiyeiy slalvtwrdc lfrplnkqvt 181 navlklieke rngetintrl isgvvqsyve lglneddafa kgptltvyke sfesqfladt 241 erfytreste flqqnpvtey mkkaearlle eqrrvqvylh estqdelark ceqvliekhl 301 eifhtefqnl ldadknedlg rmynlvsriq dglgelkkll ethihnqgla aiekcgeaal 361 ndpkmyvqtv ldvhkkynal vmsafnndag fvaaldkacg rfinnnavtk maqssskspe 421 llarycdsll kkssknpeea eledtlnqvm vvfkyiedkd vfqkfyakml akrlvhqnsa 481 sddaeasmis klkqacgfey tsklqrmfqd igvskdlneq fkkhltnsep ldldfsiqvl 541 ssgswpfqqs ctfalpsele rsyqrftafy asrhsgrklt wlyqlskgel vtncfknryt 601 lqastfqmai llqynteday tvqqltdstq ikmdilaqvl qillkskllv ledenanvde 661 velkpdtlik lylgyknkkl rvninvpmkt eqkqeqetth knieedrkll iqaaivrimk 721 mrkvlkhqql lgevltqlss rfkprvpvik kcidilieke ylervdgekd tysyla
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology.
Purification Delipidation and defibrination.
Alternative Name Cullin 1
Background Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 1 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul1 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul1 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul1 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.Synonyms: CUL-1, CUL1, Cullin-1
Gene ID 8454
NCBI Accession NP_003583
UniProt Q13616
Research Area Apoptosis/Necrosis, Proteasome
Application Notes Western blot: This antibody reacts with human Cul1 (1/500-1/1,000, An 89.6 kDa bandcorresponding to human Cul1 is detected). Most cell lines expressing Cul1 can be used as apositive control. Immunoprecipitation: The antibody immunoprecipitates in vitro translated product andprotein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, butrather use 0.1 % SDS). ELISA (1/2,000-1/10,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85.0 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
anti-Cullin 1 (CUL1) (C-Term), (AA 742-752) antibody Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
Background publications Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Read, Brownell, Gladysheva et al.: "Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha." in: Molecular and cellular biology, Vol. 20, Issue 7, pp. 2326-33, 2000 (PubMed).

Hori, Osaka, Chiba et al.: "Covalent modification of all members of human cullin family proteins by NEDD8." in: Oncogene, Vol. 18, Issue 48, pp. 6829-34, 2000 (PubMed).

Ohta, Michel, Schottelius et al.: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, 1999 (PubMed).

Tan, Fuchs, Chen et al.: "Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha." in: Molecular cell, Vol. 3, Issue 4, pp. 527-33, 1999 (PubMed).

Michel, Xiong: "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 9, Issue 6, pp. 435-49, 1998 (PubMed).

Mathias, Johnson, Winey et al.: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

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