You are viewing an incomplete version of our website. Please click to reload the website as full version.

Cullin 2 antibody (CUL2) (C-Term)

Details for Product anti-CUL2 Antibody No. ABIN117948, Supplier: Log in to see
Antigen
  • 1300003D18Rik
  • 4932411N15Rik
  • AI327301
  • ATCUL2
  • cul2
  • Cullin-2
  • cullin-2
  • cullin 2
  • F22D16.2
  • F22D16_2
  • fj48a06
  • LOC100221330
  • mKIAA4106
  • wu:fj48a06
Epitope
C-Term, AA 733-745
38
16
15
8
4
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
118
62
36
8
7
3
2
2
1
1
1
Host
Rabbit
106
16
6
Clonality
Polyclonal
Conjugate
This Cullin 2 antibody is un-conjugated
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
101
68
38
32
24
15
12
8
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Synthetic peptide corresponding to amino acids 733-745 of Human Cul2 (C-terminus) coupled to KLH.
Sequence Human Cullin 2 (745 aa 86.9 kDa): 1 mslkprvvdf detwnklltt ikavvmleyv eratwndrfs diyalcvayp eplgerlyte 61 tkiflenhvr hlhkrvlese eqvlvmyhry weeyskgady mdclyrylnt qfikknklte 121 adlqygyggv dmneplmeig elaldmwrkl mveplqaili rmllreiknd rggedpnqkv 181 ihgvinsfvh veqykkkfpl kfyqeifesp fltetgeyyk qeasnllqes ncsqymekvl 241 grlkdeeirc rkylhpssyt kvihecqqrm vadhlqflha echniirqek kndmanmyvl 301 lravstglph miqelqnhih deglratsnl tqenmptlfv esvlevhgkf vqlintvlng 361 dqhfmsaldk altsvvnyre pksvckapel lakycdnllk ksakgmtene vedrltsfit 421 vfkyiddkdv fqkfyarmla krlihglsms mdseeamink lkqacgyeft sklhrmytdm 481 svsadlnnkf nnfiknqdtv idlgisfqiy vlqagawplt qapsstfaip qeleksvqmf 541 elfysqhfsg rkltwlhylc tgevkmnylg kpyvamvtty qmavllafnn setvsykelq 601 dstqmnekel tktikslldv kminhdseke didaessfsl nmnfsskrtk fkittsmqkd 661 tpqemeqtrs avdedrkmyl qaaivrimka rkvlrhnali qevisqsrar fnpsismikk 721 cievlidkqy iersqasade ysyva
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 2. Cross reactivity with Cul2 from other sources has not been determined.
Purification Delipidation and defibrination.
Alternative Name Cullin-2 (CUL2 Antibody Abstract)
Background Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.Synonyms: CUL-2, CUL2
Gene ID 8453
NCBI Accession NP_001185706
UniProt Q13617
Application Notes Western blot: This antibody reacts with human Cul2 (1/500-1/1,000, An 86.9 kDa bandcorresponding to human Cul2 is detected). Most cell lines expressing Cul2 can be used asa positive control). Immunoprecipitation: The antibody immunoprecipitates in vitro translated product andprotein from cell lysates (using HeLa or NIH-3T3). ELISA (1/2,000-1/10,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85.0 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for up to one month or at -20 °C for longer.
Supplier Images
 image for anti-Cullin 2 antibody (CUL2) (C-Term) (ABIN117948) Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifier...
Background publications Nau: "[On commotion and on prostitution]." in: Revue médicale suisse, Vol. 6, Issue 270, pp. 2156-7, 2010 (PubMed).

Weck, Pause, Pinzer: "[Spinal meningioma as differential diagnosis of diabetic polyneuropathy]" in: Deutsche medizinische Wochenschrift (1946), Vol. 126, Issue 20, pp. 590-2, 2001 (PubMed).

Feng, Zhong, Punkosdy, Gu, Zhou, Seabolt, Kipreos: "CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans." in: Nature cell biology, Vol. 1, Issue 8, pp. 486-92, 2000 (PubMed).

Kamura, Sato, Iwai, Czyzyk-Krzeska, Conaway, Conaway: "Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 19, pp. 10430-5, 2000 (PubMed).

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Wada, Yeh, Kamitani: "Identification of NEDD8-conjugation site in human cullin-2." in: Biochemical and biophysical research communications, Vol. 257, Issue 1, pp. 100-5, 1999 (PubMed).

Ohta, Michel, Schottelius, Xiong: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, 1999 (PubMed).

Michel, Xiong: "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 9, Issue 6, pp. 435-49, 1998 (PubMed).

Mathias, Johnson, Winey, Adams, Goetsch, Pringle, Byers, Goebl: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Pause, Lee, Worrell, Chen, Burgess, Linehan, Klausner: "The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 6, pp. 2156-61, 1997 (PubMed).

Kipreos, Lander, Wing, He, Hedgecock: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).