Cullin 7 antibody (C-Term)

Catalog No. ABIN117952

Product Details anti-Cullin 7 Antibody

Target: Cullin 7 (CUL7)

Binding Specificity: AA 1679-1698, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse Cullin 7. Cross reactivity with other human cullins may occur.

Purification: Delipidation and defibrination.

Immunogen: Synthetic peptide corresponding to amino acids 1679-1698 of Human Cul7 (C-terminus) coupled coupled to KLH.

Application Details

Application Notes: Western blot (1/500-1/1,000): This antibody reacts with human Cul7, An 191.2 kDa bandcorresponding to human Cul7 is detected, Most cell lines expressing Cul7 can be used as apositive control. Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andalso protein from cell lysates (using HeLa or NIH-3T3 and others. This antibody alsoco-immunoprecipitates associated proteins in overexpressed CUL7 systems). ELISA: 1/2,000-1/10,000 Dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85.0 mg/mL (by Refractometry)

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for Cullin 7

Target: Cullin 7 (CUL7)

Alternative Name: Cullin-7 (CUL7 Products)

Synonyms: cullin-7 antibody, CUL7 antibody, Cullin-7 antibody, KIAA0076 antibody, dJ20C7.5 antibody, 2510004L20Rik antibody, AA409809 antibody, C230011P08Rik antibody, p185 antibody, p193 antibody, cullin 7 antibody, CUL7 antibody, Cul7 antibody

Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.Synonyms: CUL-7, CUL7, KIAA0076

Gene ID: 9820

NCBI Accession: NP_001161842

UniProt: Q14999

Pathways: ER-Nucleus Signaling