DDB1 antibody (AA 198-213)

Catalog No. ABIN117954

Product Details anti-DDB1 Antibody

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Binding Specificity: AA 198-213

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: Human DDB1 (1140 aa 127.0. kDa): 1 msynyvvtaq kptavngcvt ghftsaedln lliakntrle iyvvtaeglr pvkevgmygk 61 iavmelfrpk geskdllfil takynacile ykqsgesidi itrahgnvqd rigrpsetgi 121 igiidpecrm iglrlydglf kvipldrdnk elkafnirle elhvidvkfl ygcqapticf 181 vyqdpqgrhv ktyevslrek efnkgpwkqe nveaeasmvi avpepfggai iigqesityh 241 ngdkylaiap piikqstivc hnrvdpngsr yllgdmegrl fmlllekeeq mdgtvtlkdl 301 rvellgetsi aecltyldng vvfvgsrlgd sqlvklnvds neqgsyvvam etftnlgpiv 361 dmcvvdlerq gqgqlvtcsg afkegslrii rngigiheha sidlpgikgl wplrsdpnre --- 661 ssnhklvfsn vnlkevnymc plnsdgypds lalannstlt igtideiqkl hirtvplyes 721 prkicyqevs qcfgvlssri evqdtsggtt alrpsastqa lsssvssskl fssstaphet 781 sfgeevevhn lliidqhtfe vlhahqflqn eyalslvsck lgkdpntyfi vgtamvypee 841 aepkqgrivv fqysdgklqt vaekevkgav ysmvefngkl lasinstvrl yewttekdvr 901 tecnhynnim alylktkgdf ilvgdlmrsv lllaykpmeg nfeeiardfn pnwmsaveil 961 dddnflgaen afnlfvcqkd saattdeerq hlqevglfhl gefvnvfchg slvmqnlget 1021 stptqgsvlf gtvngmiglv tslseswynl lldmqnrlnk viksvgkieh sfwrsfhter 1081 ktepatgfid gdliesfldi srpkmqevva nlqyddgsgm kreataddli kvveeltrih

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.

Purification: Delipidation and defibrination.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH.

Application Details

Application Notes: This antibody reacts with human and mouse DDB1 by Western blot (1: 500 - 1: 1,000) andImmunoprecipitation. The antibody immunoprecipitates in vitro translated protein andprotein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation ofrelated proteins has not been tested. A 127.0 kDa band corresponding to human DDB1 isdetected. Most cell lines expressing DDB1 can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Alternative Name: DDB1 / XAP1 (DDB1 Products)

Synonyms: DDBA antibody, UV-DDB1 antibody, XAP1 antibody, XPCE antibody, XPE antibody, XPE-BF antibody, DDB1 antibody, xpe antibody, ddba antibody, xap1 antibody, xpce antibody, xpe-bf antibody, uv-ddb1 antibody, ddb1 antibody, 127kDa antibody, AA408517 antibody, p127-Ddb1 antibody, damage specific DNA binding protein 1 antibody, damage-specific DNA binding protein 1, 127kDa antibody, DNA damage-binding protein 1 antibody, damage-specific DNA binding protein 1 antibody, DDB1 antibody, ddb1 antibody, LOC100187356 antibody, Ddb1 antibody, ddb-1 antibody

Background: DDB1 is also known as damage-specific DNA binding protein 1, DDB p127 subunit, DDBa, UV damaged DNA-binding protein 1, UV-DDB 1, Xeroderma pigmentosum group E complementing protein, XPCe, X-associated protein 1 and XAP-1. The DDB1 gene encodes the large subunit (p127) of DNA damage-binding protein, which is a heterodimer, composed of a large and a small subunit (p48 DDB2). This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients.Synonyms: DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-DDB 1, UV-damaged DNA-binding factor, XAP-1, XPCe, XPE-BF, Xeroderma pigmentosum group E-complementing protein

Gene ID: 100290337

NCBI Accession: NP_001914

UniProt: Q16531

Pathways: DNA Damage Repair