DDB2 antibody (C-Term)

Catalog No. ABIN117955

Product Details anti-DDB2 Antibody

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Binding Specificity: AA 419-427, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: 1 mapkkrpetq ktseivlrpr nkrsrsplel epeakklcak gsgpsrrcds dclwvglagp 61 qilppcrsiv rtlhqhklgr aswpsvqqgl qqsflhtlds yrilqkaapf drratslawh 121 pthpstvavg skggdimlwn fgikdkptfi kgigaggsit glkfnplntn qfyassmegt 181 trlqdfkgni lrvfassdti niwfcsldvs assrmvvtgd nvgnvillnm dgkelwnlrm 241 hkkkvthval npccdwflat asvdqtvkiw dlrqvrgkas flyslphrhp vnaacfspdg 301 arllttdqks eirvysasqw dcplgliphp hrhfqhltpi kaawhprynl ivvgrypdpn 361 fksctpyelr tidvfdgnsg kmmcqlydpe ssgisslnef npmgdtlasa mgyhiliwsq 421 eeartrk

Specificity: This antibody reacts with DDB2.

Purification: Processed by delipidation and defibrination followed by sterile filtration

Immunogen: Synthetic peptide corresponding to amino acids 419-427 of Human DDB2 (C-terminal) coupled to KLH Genename: DDB2

Application Details

Application Notes: Western blot (1/500-1/1,000 - a 47.8 kDa band corresponding to human DDB2 is detected. Most cell lines expressing DDB2 can be used as a positive control). ELISA (1/2,000-1/10,000). Immunoprecipitation (The antibody immunoprecipitates in vitro translated protein andprotein from cell lysates (using 293T, and others). Coimmunoprecipitation of relatedproteins has not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85.0 mg/mL (by refractometry)

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DDB2 antibody (ABIN117955)

Luijsterburg, Goedhart, Moser, Kool, Geverts, Houtsmuller, Mullenders, Vermeulen, van Driel: "Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC." in: Journal of cell science, Vol. 120, Issue Pt 15, pp. 2706-16, (2007) (PubMed).

Target Details for DDB2

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Alternative Name: DDB2 (DDB2 Products)

Synonyms: 2610043A19Rik antibody, si:dkey-45f10.3 antibody, DDBB antibody, UV-DDB2 antibody, damage specific DNA binding protein 2 antibody, damage-specific DNA binding protein 2 antibody, DDB2 antibody, ddb2 antibody, Ddb2 antibody

Background: The DDB2 gene encodes the small subunit (p48) of DNA damage-binding protein, which is a heterodimer, composed of a large (p127 DDB1) and a small subunit. The DDB2 subunit appears to be required for DNA binding. This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the large subunit.Synonyms: DDB p48 subunit, DDBb, DNA damage-binding protein 2, Damage-specific DNA-binding protein 2, UV-DDB2, UV-damaged DNA-binding protein 2

Gene ID: 1643

NCBI Accession: NP_000098

UniProt: Q92466

Pathways: DNA Damage Repair