RIT2 antibody (C-Term)

Catalog No. ABIN117958

Product Details anti-RIT2 Antibody

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Binding Specificity: AA 102-113, C-Term

Reactivity: Human, Mouse, C. elegans, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIT2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Sequence: Human ROC2 (113 aa 12.6 kDa) 1 madvedgeet calashsgss gsksggdkmf slkkwnavam wswdvecdtc aicrvqvmda 61 clrcqaenkq edcvvvwgec nhsfhnccms lwvkqnnrcp lcqqdwvvqr igk

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC2. Cross reactivity may also occur with ROC2 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and APC11.

Purification: Delipidation and defibrination.

Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.

Application Details

Application Notes: This antibody reacts with human ROC2 by Western blot (1: 500-1: 1,000) andImmunoprecipitation. The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins does occur. A 12.6 kDa band corresponding tohuman ROC2 is detected. Most cell lines expressing ROC2 can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -80 °C for longer.

References for anti-RIT2 antibody (ABIN117958)

Kim, Choi, Park, Han, Kim, Chu, Kim: "Endometrial stromal sarcoma of the small bowel: a case report and review of literature." in: Annals of diagnostic pathology, Vol. 12, Issue 2, pp. 128-33, (2008) (PubMed).

Target Details for RIT2

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Alternative Name: ROC2 (RIT2 Products)

Synonyms: RIBA antibody, RIN antibody, ROC2 antibody, Rin antibody, Rit1 antibody, Roc2 antibody, RIT2 antibody, Ras like without CAAX 2 antibody, Ras-like without CAAX 2 antibody, RIT2 antibody, Rit2 antibody

Background: ROC1 also known as RING-box protein 2, Rbx2, RING finger protein 7, Regulator of cullins 2, CKII betabinding protein 1, and CKBBP1, is a probable component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC2 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. ROC2 may play a role in protecting cells from apoptosis induced by redox agents. ROC2 has a cytoplasmic and nuclear localization and is expressed in heart, liver, skeletal muscle and pancreas tissues, and at very low levels in brain, placenta and lung. 1,10-phenanthroline induces ROC2 expression. The RING-type zinc finger domain is essential for ubiquitin ligase activity. Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.Synonyms: GL2-2, GLABRA 2-like homeobox protein 2, HD-ZIP protein ROC2, Homeobox-leucine zipper protein ROC2, Homeodomain transcription factor ROC2, Protein RICE OUTERMOST CELL-SPECIFIC 2

Gene ID: 4337074

NCBI Accession: NP_001053951

UniProt: Q0J9X2

Pathways: Neurotrophin Signaling Pathway