S-Phase Kinase-Associated Protein 1 (SKP1) (C-Term), (AA 152-163) antibody

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Antigen
Synonyms
EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A, SKP1, 15kDa, Tceb1l, p19Skp1, 2610043E24Rik, 2610206H23Rik, Skp1a, ARABIDOPSIS SKP1 HOMOLOGUE 1, ASK1, ATSKP1, S phase kinase-associated protein 1, T4O12.17,  ... show more
EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A, SKP1, 15kDa, Tceb1l, p19Skp1, 2610043E24Rik, 2610206H23Rik, Skp1a, ARABIDOPSIS SKP1 HOMOLOGUE 1, ASK1, ATSKP1, S phase kinase-associated protein 1, T4O12.17, T4O12_17, UFO INTERACTING PROTEIN 1, UIP1, skp1a, zgc:73186, ocp2, emc19, ocp-ii, tceb1l, ocp-2 show less
Epitope
C-Term, AA 152-163
(9), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(66), (25), (23)
Host
Rabbit
(52), (16)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(55), (32), (19), (18), (10), (8), (7), (6), (6), (2), (2), (2), (1), (1)
Pubmed 13 references available
Catalog no. ABIN117959
Quantity 0.1 mL
Price
390.50 $   Plus shipping costs $45.00
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Immunogen Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.
Sequence Human SKP1 (163 aa 18.5 kDa) 1 mpsiklqssd geifevdvei akqsvtiktm ledlgmddeg dddpvplpnv naailkkviq 61 wcthhkddpp ppeddenkek rtddipvwdq eflkvdqgtl felilaanyl dikglldvtc 121 ktvanmikgk tpeeirktfn ikndfteeee aqvrkenqwc eek
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human SKP1. Cross reactivity is expected against mouse SKP1 based on a high degree of sequence homology. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known.
Purification Delipidation and defibrination.
Alternative Name SKP1 / OCP2
Background SKP1 is also known as S-phase kinase-associated protein 1A, Cyclin A/CDK2-associated protein p19, p19A, p19skp1, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-II protein, OCP-2, Transcription elongation factor B, and SIII. SKP1 is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, SKP1 serves as an adapter that links the F-box protein to CUL1. SKP1 interacts directly with CUL1 and F-box proteins, such as BTRC and SKP2, in the SCF complex. SKP1 also interacts with FBX29 and the cyclin A/CDK2 complex. SKP1 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8 and is also a component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X.
Alternate names: Cyclin A/CDK2-associated protein p19, EMC19, OCP-2, OCP-II, Organ of Corti protein 2, RNA polymerase II elongation factor-like protein, S-phase kinase-associated protein 1, SKP1A, TCEB1L, Transcription elongation factor B, p19A, p19skp1
Gene ID 6500
NCBI Accession NP_008861
UniProt P63208
Application Notes ELISA (1/2,000-1/10,000). This antibody reacts with human SKP1 by Western blot(1/500-1/1,000) and Immunoprecipitation. The antibody Immunoprecipitates in vitrotranslated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band correspondingto human SKP1 is detected. Most cell lines expressing SKP1 can be used as a positivecontrol.
Restrictions For Research Use only
Format Liquid
Concentration 85.0 mg/mL (by Refractometry)
Storage 4 °C
Storage Comment Store undiluted at 2-8°C.
Expiry Date 12 months
Supplier Images
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (C-Term), (AA 152-163) antibody Conjugation pathways for ubiquitin and ubiquitin-like m(UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
Background publications Sowden, Morrison, Schofield et al.: "A novel cDNA with homology to an RNA polymerase II elongation factor maps to human chromosome 5q31 (TCEB1L) and to mouse chromosome 11 (Tceb1l)." in: Genomics, Vol. 29, Issue 1, pp. 145-51, 1996 (PubMed).

Liang, Chen, Asher et al.: "Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qter." in: Gene, Vol. 184, Issue 2, pp. 163-7, 1997 (PubMed).

Feldman, Correll, Kaplan et al.: "A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p." in: Cell, Vol. 91, Issue 2, pp. 221-30, 1997 (PubMed).

Michel, Xiong: "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 9, Issue 6, pp. 435-49, 1998 (PubMed).

Ohta, Michel, Schottelius et al.: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, 1999 (PubMed).

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Schulman, Carrano, Jeffrey et al.: "Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex." in: Nature, Vol. 408, Issue 6810, pp. 381-6, 2000 (PubMed).

Matsuzawa, Reed: "Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses." in: Molecular cell, Vol. 7, Issue 5, pp. 915-26, 2001 (PubMed).

Dias, Dolios, Wang et al.: "CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16601-6, 2002 (PubMed).

Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).

Bulavin, Demidenko, Phillips et al.: "Phosphorylation of Xenopus Cdc25C at Ser285 interferes with ability to activate a DNA damage replication checkpoint in pre-midblastula embryos." in: Cell cycle (Georgetown, Tex.), Vol. 2, Issue 3, pp. 263-6, 2003 (PubMed).

Donovan, Reed: "Germline exclusion of Cks1 in the mouse reveals a metaphase I role for Cks proteins in male and female meiosis." in: Cell cycle (Georgetown, Tex.), Vol. 2, Issue 4, pp. 275-6, 2003 (PubMed).

General Wang, He, Wang et al.: "[Impact of Donor KIR2DS5 Genotype on Outcome Following Haploidentical Hematopoietic Stem Cell Transplantation.]" in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 1, pp. 111-5, 2008 (PubMed).

Hosts (52), (16)
Reactivities (66), (25), (23)
Applications (55), (32), (19), (18), (10), (8), (7), (6), (6), (2), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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