Invertase antibody (Biotin)

Antigen: Invertase

Clonality: Polyclonal

Host: Goat

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN117967

Additional Information

Characteristics: Synonyms: Beta-fructofuranosidase, Saccharase, INV1

Gene ID: 2896726

Swiss-Prot: Q9Y746

Immunogen: Invertase [Candida]

Description: Invertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta Dfructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrialand confectionary applications use yeast invertase to split sucrose into fructose andglucose and also to improve the shelf life of food products.

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin,anti-Goat Serum as well as purified and partially purified Invertase [Candida species].

Application Details

Application Notes: This product has been assayed against 1.0 µg of Invertase in a standard capture ELISAusing Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitutionconcentration is suggested for this product. Recommended Dilutions: ELISA: 1/5,000-1/25,000Western Blot: 1/500-1/3,000Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) asstabilizer.Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar Ratio: 10-20 BAC molecules per G

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. This product is stable for one month at 2-8°C as anundiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contentsand freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Enzymes, Metabolism

Restrictions: For Research Use only

Publications

Publications: Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).