Insulin antibody

Catalog No. ABIN117970

Product Details anti-Insulin Antibody

Target: Insulin (INS)

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Sequence: Human Proinsulin (precursor) 21 aa A chain 90-110. 30 aa B chain 25-54. 1 MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED 61 LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN

Specificity: This monoclonal 2D11.H5 antibody recognizes a polypeptide which is identified as Insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. This protein A purified Mouse monoclonal antibody reacts specifically with Insulin from Human and Swine sources. Cross reactivity with Insulin from Mouse and Rat does not occur. Cross reactivity with Insulin from other sources has not been determined.
Cellular Localization: Cytoplasmic.Cellular Localization:

Purification: Protein A Chromatography

Immunogen: Purified human Insulin coupled to Bovine Serum Albumin (BSA).

Clone: 2D11-H5

Isotype: IgG1

Application Details

Application Notes: This antibody is suitable for ELISA, Immunohistochemistry, Immunoblotting andimmunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or β-cellsfrom islets of Langerhans can be used as a positive control. Generally a 1: 50 to 1: 200 dilution is suitable for most applications. A 30 to 60 minuteincubation time is usually sufficient. However, depending upon the fixation conditions andthe staining system employed, optimal incubation should be determined by the user. Frozen sections of paraffin embedded tissues are recommended for preparations to use forimmunohistochemistry. No special pretreatment is required. Researchers shoulddetermine the optimal dilution for other specific assays and cell types. ELISA: Results of Mab anti-Insulin antibody were tested against human insulin by ELISA. Each well was coated with 0.1g of conjugate. The starting concentration of antibody in thedilution series was 10 μg/mL. Each point on the Y-axis represents a 3-fold dilution. Themidpoint of the titration curve represents approx-imately 5 ng/mL antibody or a 1: 200,000dilution from the stock concentration. HRP conjugated Gt-a- Mouse IgG H&L and TMBsubstrate were used for detection (see figure 1).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin (INS Products)

Synonyms: IDDM2 antibody, ILPR antibody, IRDN antibody, MODY10 antibody, ins1 antibody, xins antibody, ins1-a antibody, Insulin antibody, AA986540 antibody, Ins-2 antibody, InsII antibody, Mody antibody, Mody4 antibody, proinsulin antibody, zgc:109842 antibody, igf2-A antibody, ins antibody, ins-a antibody, ins-b antibody, insulin antibody, insulin precursor antibody, insulin II antibody, preproinsulin antibody, insulin L homeolog antibody, insulin S homeolog antibody, INS antibody, INS-IGF2 antibody, ins antibody, Ins antibody, PIN antibody, Ins2 antibody, ins.L antibody, ins.S antibody

Background: Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as β-cell and tumor (insulinoma) markers.Synonyms: INS

Molecular Weight: 6 kDa

Gene ID: 3630

NCBI Accession: NP_000198

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation