MAD2L1 antibody (AA 3-13)

Catalog No. ABIN117979

Product Details anti-MAD2L1 Antibody

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Binding Specificity: AA 3-13

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD2L1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: Human MAD2L1, 205 amino acids, predicated MW 23.5 kDa. 1 malqlsreqg itlrgsaeiv aeffsfgins ilyqrgiyps etftrvqkyg ltllvttdle 61 likylnnvve qlkdwlykcs vqklvvvisn iesgevlerw qfdiecdkta kddsapreks 121 qkaiqdeirs virqitatvt flpllevscs fdlliytdkd lvvpekwees gpqfitnsee 181 vrlrsfttti hkvnsmvayk ipvnd

Specificity: This affinity purified antibody is directed against human MAD2L1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, dog, macaque, chimpanzee and gecko based on 100 % homology for the immunogen sequence. Cross reactivity with MAD2L1 may occur from mouse and chicken sources, as only a two amino acid residue change is found within the immunogen sequence (90 % positive by BLAST). Cross reactivity with MAD2L1 homologues from other sources has not been determined.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 3-13 of Human MAD2L1 protein.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 2,000-1: 10,000) and byWestern blot (1: 500-1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a predominant band at approx. 24 kDa corresponding to full length protein bywestern blotting in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.19 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Alternative Name: MAD2L1 (MAD2L1 Products)

Synonyms: HSMAD2 antibody, MAD2 antibody, MXD2 antibody, MXI antibody, bHLHc11 antibody, AA673185 antibody, cb408 antibody, zgc:112133 antibody, mad2 antibody, XMad2 antibody, MAD2L1 antibody, mitotic arrest deficient 2 like 1 antibody, MAX interactor 1, dimerization protein antibody, MAD2 mitotic arrest deficient-like 1 antibody, MAD2 mitotic arrest deficient-like 1 (yeast) antibody, mitotic arrest deficient 2 like 1 S homeolog antibody, MAD2L1 antibody, MXI1 antibody, Mad2l1 antibody, mad2l1 antibody, mad2l1.S antibody

Background: MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. This protein has a nuclear localization.Synonyms: MAD2, Mitotic spindle assembly checkpoint protein MAD2A

Gene ID: 4085

NCBI Accession: NP_002349

UniProt: Q13257