Notch 1 (cleaved N-term.) (Human) antibody

Overview


	Antigen:	Notch 1 (cleaved N-term.)
	Antibody Type:	Polyclonal
	Application:	Enzyme Immunoassay (EIA), Western Blotting (WB)
	Reactivity:	Human
	Host:	Rabbit
	Quantity:	0.2 ml (90 mg/ml (by Refractometry))
	Price:	340,00 € (Plus shipping costs and VAT)
	Order number:	ABIN117996
	Availability:	Ships in 3 to 10 days

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Additional Information: Notch 1 (cleaved N-term.) (Human) antibody

Antigen	Notch 1 (cleaved N-term.)
Immunogen	This whole rabbit serum was prepared by repeated immunizations with a speptide corresponamino acid residues 1755-1767 of human Notch 1. This is the N-terminal sequence of the cleaved N intracellular domain (NICD). A residue of cysteine was added to the amino terminal end to facilitatcoupling. ynthetic ding to e Peptide Seq.: V-L-L-S-R-K-R-R-R-Q-H-G-Q-C
Reactivity	Human
Antibody Type	Polyclonal
Format	Serum
Host	Rabbit
Specificity	This antiserum is directed against human NOTCH 1. Based on sequence we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

Application Details: Notch 1 (cleaved N-term.) (Human) antibody

Application	Enzyme Immunoassay (EIA), Western Blotting (WB)
Application Notes	Western blotting: This product was assayed by immunoblot against recombinant mouse Notch constructs in 293 cells. An 80 kDa band corresponding to Notch 1 was observed at a 1:500 dilution. ELISA: This product was also assayed against the peptide immunogen in a standard sandwich ELISA using Peroxidase conjugated anti-Rabbit IgG [H&,L] (Goat) and ABT (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:60,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Concentration	90 mg/ml (by Refractometry)
Buffer	Liquid (sterile filtered) , 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.1% (w/v) Sodium Azide as preservative.
Storage	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Research Area	Receptors, Signaling, Inflammation, Immunology
Restrictions	Research only

Publications: Notch 1 (cleaved N-term.) (Human) antibody

Publications

Bertrand, Eckfeldt, Lysholm et al.:
"Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells."
In: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K , Vol. 14, Issue 12, pp. 2095-102, 2001/01 (PubMed)

Images: Notch 1 (cleaved N-term.) (Human) antibody

Image for Notch 1 (cleaved N-term.) (Human) antibody (1)

External Information: Notch 1 (cleaved N-term.) (Human) antibody

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