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Notch 1 (NOTCH1) (AA 1755-1767), (N-Term) antibody

Details for Product No. ABIN117996
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Antigen
Synonyms notch1, NOTCH1, hn1, tan1, notch, xotch, xnotch, notch-1, xnotch1, TAN1, hN1, 9930111A19Rik, Mis6, N1, Tan1, lin-12, NOTCH, Xotch, notch1-a
Epitope
AA 1755-1767, N-Term
(29), (19), (17), (7), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(116), (90), (30), (3), (2), (1), (1), (1), (1)
Host
Rabbit
(88), (75), (5), (4), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (8), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(109), (52), (46), (36), (33), (26), (16), (10), (6), (2), (1), (1)
Pubmed 2 references available
Quantity 0.2 mL
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Catalog No. ABIN117996
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Immunogen Synthetic peptide corresponding to amino acid residues 1755-1767 of Human Notch1.
Sequence V-L-L-S-R-K-R-R-R-Q-H-G-Q-C Remarks: This is the N-terminal sequence of the cleaved N intracellular domain (NICD). A residue of cysteine was added to the amino terminal end to facilitate coupling.
Specificity This antiserum is directed against human NOTCH 1. Based on sequence we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Purification Serum
Alternative Name NOTCH1
Background Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Alternate names: Neurogenic locus notch homolog protein 1, Notch 1, TAN1, hN1
Gene ID 4851
NCBI Accession NP_060087.3
UniProt P46531
Research Area Receptors, Signaling, Inflammation, Immunology
Application Notes Western blotting: 1/500-1/2,000. This product was assayed by immunoblot againstrecombinant mouse Notch constructs in 293 cells. An 80 kDa band corresponding to Notch1 was observed at a 1/500 dilution. An 80 kDa band corresponding to Notch 1 wasobserved at a 1: 500 dilution. ELISA: 1/20,000-1/60,000. This product was also assayed against the peptide immunogenin a standard Sandwich ELISA using Peroxidase conjugated Goat anti-Rabbit IgG [H&L](Cat#R1364HRP) and ABT (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL (by Refractometry)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Notch 1 (NOTCH1) (AA 1755-1767), (N-Term) antibody Figure. Immunoblotting. Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot. Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods. A reaction with diluted primary antibody was followed by washing, reaction with a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122), and color development. Lane M: Mol wt markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)- myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)- myc. Personal communication, Dr. Stacey Huppert.
Background publications Bertrand, Eckfeldt, Lysholm et al.: "Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells." in: Leukemia, Vol. 14, Issue 12, pp. 2095-102, 2001 (PubMed).

Gridley: "Notch signaling and inherited disease syndromes." in: Human molecular genetics, Vol. 12 Spec No 1, pp. R9-13, 2003 (PubMed).

Validation Images
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