Notch 1 (NOTCH1) (AA 2488-2502) antibody

Details for Product No. ABIN117997
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Synonyms notch1, NOTCH1, hn1, tan1, notch, xotch, xnotch, notch-1, xnotch1, TAN1, hN1, 9930111A19Rik, Mis6, N1, Tan1, lin-12, NOTCH, Xotch, notch1-a
AA 2488-2502
(30), (19), (17), (7), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(120), (102), (31), (4), (2), (2), (2), (1), (1)
(90), (77), (11), (5), (4), (3), (2)
(10), (9), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(113), (63), (47), (39), (36), (28), (16), (10), (8), (2), (1), (1)
Pubmed 2 references available
Quantity 0.2 mL
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Catalog No. ABIN117997
390.50 $
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Immunogen Synthetic peptide corresponding to amino acid residues 2488-2502 of Human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Sequence C-Q-H-S-Y-S-S-P-V-D-N-T-P-S-H-Q
Specificity This antiserum is directed against Human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Species Reactivity: Tested: Human.
Purification Serum
Alternative Name NOTCH1
Background Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
Alternate names: Neurogenic locus notch homolog protein 1, Notch 1, TAN1, hN1
Gene ID 4851
NCBI Accession NP_060087
UniProt P46531
Research Area Receptors, Signaling, Inflammation, Immunology
Application Notes Preliminary experiments show reactivity at a 1: 1000 dilution by Western blot. A strongresponse was detected by ELISA against the immunizing peptide. Recommended dilutions: ELISA: 1/20,000-1/100,000Western blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 75 mg/mL (by refractometry)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Bertrand, Eckfeldt, Lysholm et al.: "Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells." in: Leukemia, Vol. 14, Issue 12, pp. 2095-102, 2001 (PubMed).

Gridley: "Notch signaling and inherited disease syndromes." in: Human molecular genetics, Vol. 12 Spec No 1, pp. R9-13, 2003 (PubMed).

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