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Notch1 antibody (Notch 1) (AA 2488-2502)

Details for Product anti-NOTCH1 Antibody No. ABIN117997, Supplier: Log in to see
Antigen
  • notch1
  • NOTCH1
  • hn1
  • tan1
  • notch
  • xotch
  • xnotch
  • notch-1
  • xnotch1
  • TAN1
  • hN1
  • 9930111A19Rik
  • Mis6
  • N1
  • Tan1
  • lin-12
  • NOTCH
  • Xotch
  • notch1-a
Alternatives
anti-Human Notch1 antibody for Western Blotting
Epitope
AA 2488-2502
50
36
33
17
17
10
8
7
7
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
198
150
60
6
2
2
2
1
1
Host
Rabbit
134
127
15
8
7
1
1
Clonality
Polyclonal
Conjugate
This Notch1 antibody is un-conjugated
23
14
13
7
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
179
89
75
74
50
35
26
16
15
5
3
2
2
2
2
2
2
1
1
1
1
Supplier
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Immunogen Synthetic peptide corresponding to amino acid residues 2488-2502 of Human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Sequence C-Q-H-S-Y-S-S-P-V-D-N-T-P-S-H-Q
Specificity This antiserum is directed against Human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Serum
Alternative Name NOTCH1 (NOTCH1 Antibody Abstract)
Background Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.Synonyms: Neurogenic locus notch homolog protein 1, Notch 1, TAN1, hN1
Gene ID 4851
NCBI Accession NP_060087
UniProt P46531
Research Area Receptors, Signaling, Inflammation, Immunology
Pathways Notch Signaling
Application Notes Preliminary experiments show reactivity at a 1: 1000 dilution by Western blot. A strongresponse was detected by ELISA against the immunizing peptide. Recommended dilutions: ELISA: 1/20,000-1/100,000Western blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 75 mg/mL (by refractometry)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 and 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Gridley: "Notch signaling and inherited disease syndromes." in: Human molecular genetics, Vol. 12 Spec No 1, pp. R9-13, 2003 (PubMed).

Bertrand, Eckfeldt, Lysholm et al.: "Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells." in: Leukemia, Vol. 14, Issue 12, pp. 2095-102, 2001 (PubMed).