ANAPC10 antibody (N-Term)

Catalog No. ABIN118001

Product Details anti-ANAPC10 Antibody

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC10 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with APC10.

Purification: Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Immunogen: A synthetic peptide corresponding to amino acids near the amino terminus of human APC10

Application Details

Application Notes: ELISA 1: 15,000 - 1: 80,000Western blot 1: 500 - 1: 1,500Immunohistochemistry 1: 250 - 1: 1,000
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.5 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ANAPC10

Target: ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Alternative Name: APC10 / ANAPC10 (ANAPC10 Products)

Synonyms: APC10 antibody, DOC1 antibody, 1500026N15Rik antibody, A830003M23Rik antibody, CG11419 antibody, Dmel\\CG11419 antibody, ANAPC10 antibody, SPBC1E8.06 antibody, anapc10 antibody, MGC85037 antibody, DDBDRAFT_0202332 antibody, DDBDRAFT_0235160 antibody, DDB_0202332 antibody, DDB_0235160 antibody, anaphase promoting complex subunit 10 antibody, Anaphase Promoting Complex subunit 10 antibody, anaphase promoting complex subunit DOC1 antibody, anaphase-promoting complex subunit Apc10 antibody, anaphase promoting complex subunit antibody, predicted protein antibody, anaphase promoting complex subunit 10 S homeolog antibody, ANAPC10 antibody, Anapc10 antibody, APC10 antibody, DOC1 antibody, apc10 antibody, CAALFM_C107850CA antibody, anapc10 antibody, LOC692856 antibody, anapc10.S antibody, NAEGRDRAFT_66759 antibody

Background: APC (Anaphase-Promoting Complex or Cyclosome) is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds1/Cut2 and cyclin B. It was found that APC10/Doc1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid-body in telophase and cytokinesis. These results strongly support the notion that human APC10/Doc1 may be one of the APC core subunits rather than the transiently associated regulatory factor.Synonyms: Anaphase-promoting complex subunit 10, Cyclosome subunit 10

Gene ID: 10393

NCBI Accession: NP_055700

UniProt: Q9UM13

Pathways: M Phase