Secreted phosphoprotein 1 (SPP1) antibody

Details for Product No. ABIN118005
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Antigen
Synonyms zgc:111821, BNSP, BSPI, ETA-1, OPN, 2AR, Apl-1, Bsp, Eta, OP, Opn, Opnl, Ric, Spp-1, OSP, AI463453, SPP, SPP1, Spph1, SPPase1
Reactivity
Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(230), (63), (41), (17), (15), (13), (3), (1), (1)
Host
Rabbit
(184), (80), (11), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(11), (9), (8), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(218), (144), (67), (65), (23), (20), (18), (15), (12), (10), (9), (8), (3), (2), (2), (1), (1), (1), (1)
Pubmed 12 references available
Quantity 0.2 mL
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Catalog No. ABIN118005
390.50 $
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Immunogen Synthetic peptide corresponding to Human Osteopontin conjugated to KLH using maleimide.
Sequence C-K-S-K-K-F-R-R-P-D-I-Q-Y-P-D
Specificity This antiserum is directed against Human osteopontin. The antibody recognizes the full length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment.
Purification Serum
Alternative Name Osteopontin / SPP1
Background Osteopontin (OPN) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1. Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN. OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells. It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events.Synonyms: BNSP, Bone Sialoprotein 1, Nephropontin, OPN, SPP-1, Secreted Phosphoprotein 1, Urinary Stone Protein, Uropontin
Gene ID 6696
NCBI Accession NP_000573
UniProt P10451
Research Area Hormones, Enzymes, Metabolism, Extracellular Matrix
Application Notes This antibody is suitable for Western blotting, Immunohistochemistry (Formalin FixedParaffin-Embedded Sections) and ELISA. A 1/1000 dilution will detect strongly
Restrictions For Research Use only
Format Liquid
Concentration 75 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 and 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
Supplier Images
anti-Secreted phosphoprotein 1 (SPP1) antibody Figure 1. Immunohistochemistry. Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteoponin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.
Background publications Denhardt, Chambers: "Overcoming obstacles to metastasis--defenses against host defenses: osteopontin (OPN) as a shield against attack by cytotoxic host cells." in: Journal of cellular biochemistry, Vol. 56, Issue 1, pp. 48-51, 1995 (PubMed).

Denhardt, Lopez, Rollo et al.: "Osteopontin-induced modifications of cellular functions." in: Annals of the New York Academy of Sciences, Vol. 760, pp. 127-42, 1995 (PubMed).

Weber, Cantor: "The immunology of Eta-1/osteopontin." in: Cytokine & growth factor reviews, Vol. 7, Issue 3, pp. 241-8, 1997 (PubMed).

Yamasaki, Nair, Bhattacharya et al.: "Cryptic appearance of a new clone of Vibrio cholerae serogroup 01 biotype El Tor in Calcutta, India." in: Microbiology and immunology, Vol. 41, Issue 1, pp. 1-6, 1997 (PubMed).

Welch, Birch, Makarov et al.: "Histomorphometry of distraction osteogenesis in a caprine tibial lengthening model." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 13, Issue 1, pp. 1-9, 1998 (PubMed).

Liaw, Birk, Ballas et al.: "Altered wound healing in mice lacking a functional osteopontin gene (spp1)." in: The Journal of clinical investigation, Vol. 101, Issue 7, pp. 1468-78, 1998 (PubMed).

Crawford, Matrisian, Liaw: "Distinct roles of osteopontin in host defense activity and tumor survival during squamous cell carcinoma progression in vivo." in: Cancer research, Vol. 58, Issue 22, pp. 5206-15, 1998 (PubMed).

Yoshitake, Rittling, Denhardt et al.: "Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 14, pp. 8156-60, 1999 (PubMed).

Nau, Liaw, Chupp et al.: "Attenuated host resistance against Mycobacterium bovis BCG infection in mice lacking osteopontin." in: Infection and immunity, Vol. 67, Issue 8, pp. 4223-30, 1999 (PubMed).

Ashkar, Weber, Panoutsakopoulou et al.: "Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity." in: Science (New York, N.Y.), Vol. 287, Issue 5454, pp. 860-4, 2000 (PubMed).

McCawley, Matrisian: "Matrix metalloproteinases: multifunctional contributors to tumor progression." in: Molecular medicine today, Vol. 6, Issue 4, pp. 149-56, 2000 (PubMed).

"In Celebration of 100 Years of Publication." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 1, 2001 (PubMed).

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