SH3BP2 antibody (pSer427)

Catalog No. ABIN118009

Product Details anti-SH3BP2 Antibody

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Binding Specificity: AA 422-433, pSer427

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3BP2 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Specificity: This product is an affinity purified antibody produced by immunoaffinity chromatography using phosphor peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human SH3BP2 protein at the pS427 residue. BLAST analysis indicates sequence variations are found within dog, chicken, mouse and rat homologues. Limited cross reactivity is expected with SH3BP2 from these sources. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 422-433 of Human SH3BP3 protein (SH3 Domain Binding Protein 2).

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 2,000-1: 10,000) andWestern blot (1: 500-1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 60 kDa in size corresponding to SH3BP2 by westernblotting in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for SH3BP2

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Alternative Name: SH3BP2 (SH3BP2 Products)

Synonyms: SH3BP2 antibody, 3BP-2 antibody, 3BP2 antibody, CRBM antibody, CRPM antibody, SH3 domain binding protein 2 antibody, SH3-domain binding protein 2 antibody, SH3BP2 antibody, Sh3bp2 antibody

Background: SH3BP2 Src Homology 3 Binding Protein 2 is also known as 3BP-2 and SH3 Binding Protein 2. The Src homology 3 (SH3) region is a small protein domain presented in a very large group of proteins, including cytoskeletal elements and signaling proteins. SH3 domains are believed to serve as modules that mediate protein-protein associations and, along with Src homology 2 (SH2) domains, regulate cytoplasmic signaling. SH3BP2 is composed of an N terminal pleckstrin homology (PH) domain, a ten aa SH3 binding domain, three modular peptide recognition domains, and a C terminal SH2 domain. SH3BP2 function relates to signal transduction and regulation. SH3BP2 binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Phosphorylation of SH3BP2 occurs on S427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase). Defects in SH3BP2 are the cause of cherubism (CRBM), an autosomal dominant inherited syndrome. It is characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.Synonyms: 3BP-2, 3BP2, SH3 domain-binding protein 2, SH3BP-2

Gene ID: 6452

NCBI Accession: NP_001116153

UniProt: P78314

Pathways: TCR Signaling