PPARD antibody (N-Term)

Catalog No. ABIN118017

Product Details anti-PPARD Antibody

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Binding Specificity: N-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARD antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This affinity purified antibody is directed against Mouse PPAR delta protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from Mouse and Rat sources based on 100 % homology for the immunogen sequence. Cross reactivity with PPAR delta protein from Human, Chimpanzee and Rabbit may occur as this sequence shows 85 % homology with the protein from these sources. Cross reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids near the amino terminus of Mouse PPAR delta.

Application Details

Application Notes: This antibody has been tested for use in ELISA (1/600,000) and Western blot(1/500-1/5,000). Expect a single band approximately 43 kDa in size corresponding to PPAR delta by Westernblot in the appropriate tissue or cell lysate.

Restrictions: For Research Use only

Handling

Concentration: 2.03 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for PPARD

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Alternative Name: PPAR-delta (PPARD Products)

Synonyms: FAAR antibody, NR1C2 antibody, NUC1 antibody, NUCI antibody, NUCII antibody, PPARB antibody, NUC-1 antibody, Nr1c2 antibody, PPAR-beta antibody, PPAR-delta antibody, PPAR[b] antibody, PPARdelta antibody, Pparb antibody, Pparb/d antibody, PPARBETA antibody, ppard antibody, pparb antibody, PPARD antibody, PPAR-D/B antibody, peroxisome proliferator activated receptor delta antibody, peroxisome proliferator activator receptor delta antibody, peroxisome proliferator activated receptor delta S homeolog antibody, peroxisome proliferator-activated receptor delta antibody, PPARD antibody, Ppard antibody, ppard.S antibody, ppard antibody

Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are liganddependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPARalpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.Synonyms: NR1C2, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, PPAR-beta, PPARB, PPARD, Peroxisome proliferator-activated receptor delta

Gene ID: 19015

NCBI Accession: NP_035275

UniProt: P35396

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation